Fabrication of micro-porous hyaluronic acid hydrogels through salt leaching
Fabrication of micro-porous hyaluronic acid hydrogels through salt leaching
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DOI：
10.1016/j.eurpolymj.2020.109870
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发表时间：
2020-07-15
影响因子：
6
通讯作者：
Rao, Shreyas S.
中科院分区：
文献类型：
--
作者：
Coogan, Kasie R.;Stone, Payton T.;Rao, Shreyas S.
用于研究细胞行为的生理学相关培养平台的开发在组织工程中以及在体外疾病模型的开发中特别令人感兴趣。透明质酸是一种存在于细胞外基质中的糖胺聚糖，其衍生物通常用于创建三维仿生水凝胶支架，用于体外细胞行为的研究。在此，我们报告了使用简单的盐浸技术制造微孔透明质酸水凝胶。致孔剂，具体地，不同尺寸的盐颗粒（即，直径< 63 μ m、63-106 μ m、106-250 μ m），以获得一系列多孔结构。的水凝胶的微观结构，其特征在于通过扫描电子显微镜，并进行流变学和溶胀研究，以进一步评估形态和机械性能。这些研究的分析表明，孔面积的增加对应于较大的致孔剂尺寸，而致孔剂尺寸对储能模量或吸水率没有显著影响。然而，多孔水凝胶表现出更大程度的溶胀和降低的储能模量相比，无孔水凝胶。特别地，106-250 μ m的最大粒径导致44.6 +/-2.28的溶胀比和54 +/-4.97 Pa的储能模量，而无孔透明质酸水凝胶分别记录到24.3 +/-2.30的溶胀比和402 +/-209 Pa的储能模量。在随后的时间点未观察到水凝胶降解的显著差异;然而，具有最大孔面积的水凝胶最初降解的程度更大。细胞相容性评估显示，在所有测试的水凝胶条件下均无细胞毒性证据。这样一个系统，结合微米级的孔隙率，可以提供一个有用的工具，用于研究细胞-基质相互作用在生理相关的设置。
Development of physiologically relevant culture platforms for studying cell behavior has been of particular interest in tissue engineering as well as in development of in vitro disease models. Hyaluronic acid is a glycosaminoglycan found in the extracellular matrix, derivatives of which are commonly used to create three-dimensional biomimetic hydrogel scaffolds for the study of cell behavior in vitro. Herein, we report the fabrication of micro-porous hyaluronic acid hydrogels using a simple salt leaching technique. Porogens, specifically, salt particles of different sizes (i.e., < 63 mu m, 63-106 mu m, 106-250 mu m in diameter), were utilized to obtain a range of porous structures. The microstructure of the hydrogels was characterized through scanning electron microscopy, and rheological and swelling studies were performed to further evaluate morphological and mechanical properties. Analysis of these studies indicated that an increase in pore area corresponded to a larger porogen size, whereas porogen size had no significant effect on storage modulus or water absorption. However, porous hydrogels exhibited a greater degree of swelling and reduced storage modulus compared to nonporous hydrogels. In particular, the largest particle size of 106-250 mu m resulted in a swelling ratio of 44.6 +/- 2.28 and storage modulus of 54 +/- 4.97 Pa versus a swelling ratio of 24.3 +/- 2.30 and storage modulus of 402 +/- 209 Pa noted for nonporous hyaluronic acid hydrogels, respectively. No significant difference in degradation of hydrogels was observed at later time points; however, hydrogels with the largest pore area degraded to a greater extent initially. Assessment of cellular compatibility showed no evidence of cytotoxicity for all hydrogel conditions tested. Such a system, incorporating micron-level porosity, could provide a useful tool for studying cell-matrix interactions in a physiologically relevant setting.