Characterization of plasma lipoproteins separated and purified by agarose-column chromatography.
Characterization of plasma lipoproteins separated and purified by agarose-column chromatography.
复制标题
通过琼脂糖柱色谱分离和纯化的血浆脂蛋白的表征。
作者：
Lawrence L. Rudel;Jason A. Lee;Manford D. Morris;James M. Felts
1.本文介绍了一种分离人血浆脂蛋白的简便方法。在该技术中，脂蛋白在d1.225通过超离心从血浆中除去，之后通过琼脂糖柱色谱法分离和纯化。2.琼脂糖柱层析后得到三个主要类别。类之间的分离是极好的;超过95%的脂蛋白从柱洗脱回收的形式纯化脂蛋白类。3.每类脂蛋白的特点是免疫学，化学，免疫学和电子显微镜。柱分离的脂蛋白的性质进行了比较，与极低密度脂蛋白，低密度脂蛋白，和高密度脂蛋白通过连续超离心分离的密度分别为1.006，1.063和1.21。4.根据每个标准，来自琼脂糖柱的峰I脂蛋白与极低密度脂蛋白相同，峰II脂蛋白与低密度脂蛋白相同，峰III脂蛋白与高密度脂蛋白相同。因此，通过两种方法分离的脂蛋白类别如果不相同，也是相似的。5.琼脂糖柱分离技术的优点是可以节省2 - 3倍的时间。此外，色谱柱洗脱模式可记录血浆中脂蛋白的大小分布。6.报告了人血浆脂蛋白的最完整表征。恒河猴和兔脂蛋白的结果相同。
1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.