Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering
Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering
复制标题
用于血管组织工程的静电纺丝硫酸化丝素蛋白纳米纤维支架
DOI：
10.1016/j.biomaterials.2011.02.002
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发表时间：
2011-05-01
期刊：
影响因子：
14
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Liu, Haifeng;Li, Xiaoming;Fan, Yubo
组织工程小直径血管移植物的主要缺点之一是无法在管腔表面获得汇合的内皮。当暴露于体内血管系统时，松散附着的内皮细胞（EC）很容易与血管壁分离。因此，血管移植物管腔表面上的任何裸露区域都可能通过血小板沉积和活化导致血栓形成。如果裸露区域能够表达抗凝活性，直到内皮细胞衬里完全形成，则可能会大大提高血管重建成功的机会。在本研究中，我们制备了硫酸化丝素蛋白纳米纤维支架（S-silk支架），并在体外评估了S-silk支架的抗凝活性和细胞相容性，以提高其抗血栓形成能力，并深入了解其在血管组织工程中的潜在用途。通过与氯磺酸在吡啶中反应制备硫酸化丝素蛋白，然后通过静电纺丝技术形成S-丝素支架。 FTIR 分析表明硫酸盐基团成功掺入丝素蛋白分子中。结果发现，与丝素蛋白纳米纤维支架（Silkscaffolds）相比，S-丝支架的抗凝血活性显着增强。血管细胞，包括 EC 和平滑肌细胞 (SMC)，表现出与 S-丝支架的牢固附着，并且增殖良好，一些表型相关标记基因和蛋白质的表达较高。总体而言，本研究的数据表明 S-丝支架与血管细胞一起使用适合开发组织工程血管移植物。 (C) 2011 Elsevier Ltd. 保留所有权利。
One of the major downfalls of tissue-engineered small-diameter vascular grafts is the inability to obtain a confluent endothelium on the lumenal surface. Loosely attached endothelial cells (ECs) are easily separated from the vessel wall when exposed to the in vivo vascular system. Thus any denuded areas on the lumenal surface of vascular grafts may lead to thrombus formation via platelet deposition and activation. If the denuded areas could express anticoagulant activity until the endothelial cell lining is fully achieved, it may greatly improve the chances of successful vascular reconstruction. In this study, we fabricate sulfated silk fibroin nanofibrous scaffolds (S-silk scaffolds) and assess the anticoagulant activity and cytocompatibility of S-silk scaffolds in vitro in order to improve the antithrombogenicity and get some insights into its potential use for vascular tissue engineering. Sulfated silk fibroin was prepared by reaction with chlorosulphonic acid in pyridine, and then was developed to form an S-silk scaffold by electrospinning technique. FTIR analyses identified the successful incorporation of sulfate groups in silk fibroin molecules. It was found that the anticoagulant activity of S-silk scaffolds was significantly enhanced compared with silk fibroin nanofibrous scaffolds (Silk scaffolds). Vascular cells, including ECs and smooth muscle cells (SMCs), demonstrated strong attachment to S-silk scaffolds and proliferated well with higher expression of some phenotype-related marker genes and proteins. Overall, the data in this study suggest the suitability of S-silk scaffolds used along with vascular cells for the development of tissue-engineered vascular grafts. (C) 2011 Elsevier Ltd. All rights reserved.