Forebrain and midbrain distribution of major benzodiazepine-sensitive GABAA receptor subunits in the adult C57 mouse as assessed with in situ hybridization
Forebrain and midbrain distribution of major benzodiazepine-sensitive GABAA receptor subunits in the adult C57 mouse as assessed with in situ hybridization
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DOI：
10.1016/j.neuroscience.2007.09.008
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发表时间：
2007-12-05
期刊：
影响因子：
3.3
通讯作者：
Ressler, K. J.
中科院分区：
文献类型：
--
作者：
Heldt, S. A.;Ressler, K. J.
在成人大脑中，GABA是主要的抑制性神经递质。最近对具有不同GABA受体亚型和相关蛋白突变的小鼠品系的研究，促进了对GABA行为和药理功能的理解。转基因小鼠已成为辨别GABA能功能的重要工具。因此，详细了解不同GABA(A)亚型受体在小鼠体内的解剖分布是了解突变小鼠正常和药物诱导行为变化的神经电路的先决条件。在本研究中，我们使用原位杂交组织化学和[S-35]UTP标记的核糖核酸探针，检测了邻近脑冠状切片(α1、α2、α3、α5、β2、β3和伽马2)中七个主要GABA(A)受体亚单位基因转录的区域表达模式。我们的结果表明，许多这些GABA能基因在成人大脑的大部分区域共表达，包括新皮质、海马体、杏仁核、丘脑和纹状体。然而，每个基因也表现出独特的区域特异性分布模式，表明不同的神经元回路可能服务于特定的生理和药物功能。爱思唯尔有限公司代表伊布罗出版。
In the adult brain, GABA is the major inhibitory neurotransmitter. Understanding of the behavioral and pharmacological functions of GABA has been advanced by recent studies of mouse lines that possess mutations in various GABA receptor subtypes and associated proteins. Genetically altered mice have become important tools for discerning GABAergic function. Thus detailed knowledge of the anatomical distribution of different GABA(A) subtype receptors in mice is a prerequisite for understanding the neural circuitry underlying changes in normal and drug-induced behaviors seen in mutated mice. In the current study, we used in situ hybridization histochemistry with [S-35]UTP-labeled ribo-probes to examine the regional expression pattern of mRNA transcripts for seven major GABA(A) receptor subunits in adjacent coronal brain sections (alpha 1, alpha 2, alpha 3, alpha 5, beta 2, beta 3, and gamma 2). Our results indicate that many of these GABAergic genes are co-expressed in much of the adult brain including the neocortex, hippocampus, amygdala, thalamus and striatum. However, each gene also shows a unique region-specific distribution pattern, indicative of distinct neuronal circuits that may serve specific physiological and pharmacological functions. Published by Elsevier Ltd on behalf of IBRO.