Modest but reproducible inhibition of human immunodeficiency virus type 1 infection in macrophages following LEDGFp75 silencing
Modest but reproducible inhibition of human immunodeficiency virus type 1 infection in macrophages following LEDGFp75 silencing
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DOI：
10.1128/jvi.02470-05
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发表时间：
2006-07-01
影响因子：
5.4
通讯作者：
Stevenson, Mario
中科院分区：
文献类型：
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作者：
Zielske, Steven P.;Stevenson, Mario
LEDGFp75是一种与人类免疫缺陷病毒1型(HIV-1)整合酶结合的细胞蛋白，具有高度的特异性和亲和力，但其在感染中的作用尚未确定。我们用野生型HIV感染LEDGFp75缺陷的原代巨噬细胞，以评估潜在的感染表型，这将为LEDGFp75的功能提供线索。将LEDGFp75沉默70%至80%导致巨噬细胞感染艾滋病毒的平均减少53%。对感染中间产物的分析表明，在LEDGFp75缺陷的巨噬细胞中，整合减少了高达74%，而不是两个长末端重复(2ltr)环或晚期cDNA。因此，LEDGFp75适度参与了巨噬细胞中HIV-1的整合。
LEDGFp75 is a cellular protein which binds human immunodeficiency virus type 1 (HIV-1) integrase with high specificity and affinity but whose function in infection has not been defined. We infected LEDGFp75-deficient primary macrophages with wild-type HIV in order to assess potential infection phenotypes which would provide clues to LEDGFp75 function. Silencing of LEDGFp75 by 70 to 80% resulted in an average of 53% reduced infection of macrophages by HIV. Analysis of infection intermediates showed that integration, but not two-long-terminal-repeat (2LTR) circles or late cDNAs, was reduced up to 74% in LEDGFp75-deficient macrophages. Therefore, LEDGFp75 has a modest involvement in HIV-1 integration in macrophages.