A membrane blotting method for following the time course of protein radioiodination using Iodobeads.
A membrane blotting method for following the time course of protein radioiodination using Iodobeads.
复制标题
一种使用 Iodobeads 跟踪蛋白质放射性碘化时间过程的膜印迹方法。
DOI：
10.1016/0003-2697(91)90527-z
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发表时间：
1991
影响因子：
2.9
通讯作者：
Rudick,MJ
中科院分区：
文献类型：
--
作者：
Cheng,H;Rudick,MJ
描述了一种监测和优化放射性碘化物掺入蛋白质的方法。固定化氯胺-T（碘珠）的使用与硝化纤维上的定时印迹相结合，构成了高效测定系统的基础。比较了 Iodobeads 和常用的可溶形式氯胺-T 的结果。
A method for monitoring and optimizing the incorporation of radioiodide into protein is described. The use of immobilized chloramine-T (Iodobeads) combined with timed blots on nitrocellulose forms the basis of an efficient assay system. Results with Iodobeads and with the commonly utilized soluble form of chloramine-T are compared.