Molecular phylogeny of Coelogyne (Epidendroideae; Orchidaceae) based on plastid RFLPS, matK, and nuclear ribosomal its sequences:: Evidence for polyphyly
Molecular phylogeny of Coelogyne (Epidendroideae; Orchidaceae) based on plastid RFLPS, matK, and nuclear ribosomal its sequences:: Evidence for polyphyly
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DOI：
10.2307/3558367
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发表时间：
2001-10-01
影响因子：
3
通讯作者：
Bachmann, K
中科院分区：
文献类型：
--
作者：
Gravendeel, B;Chase, MW;Bachmann, K
为了评价Coelogyne的单系性，揭示其与Coclogyninae亚族近缘属的亲缘关系，我们收集了42个分类群（28种Coelogyne和14个其他属的代表）和Bletiinae和Thuniinae的3个外类群的11个质体区域的PCR（polymerase chain reaction）扩增的限制性片段长度多态性（RFLP）。我们还测序了大部分质体trnK内含子（主要是matK）和核核糖体DNA内部转录间隔区ITS1和ITS2（包括5.8S基因）。单独的系统发育分析，每个数据集使用最大简约法产生的主要是全等的（除了Panisea的位置），但弱支持的分支。简约分析的组合数据清楚地确定了三个主要的分支Coclogyninae。而Coelogyninae是单系的，Coelogyne是多系的，与物种失败到至少两个良好的支持分支。在以前的分类中使用的形态特征的效用进行了探索，通过重建四个分子树之一的字符状态进化。唇基和花瓣形状相似。而子房毛被和花数与支持良好的组一致。我们的研究结果的意义进行了讨论的Coelogyne的分类。并建议将Neogyna和Pholidota包括在内，并删除几个种。
To evaluate the monophyly of Coelogyne (Epidendroideae: Orchidaceae) and reveal sectional relationships and relations to allied genera in subtribe Coclogyninae, we collected PCR (polymerase chain reaction) amplified restriction fragment length polymorphisms (RFLPs) from 11 plastid regions for 42 taxa (28 Coelogyne species and 14 representatives of other genera) and three outgroups from Bletiinae and Thuniinae. We also sequenced a large portion of the plastid trnK intron (mostly matK) and the nuclear ribosomal DNA internal transcribed spacers ITS1 and ITS2 (including the 5.8S gene). Separate phylogenetic analyses on each data set using maximum parsimony produced mainly congruent (except for the position of Panisea) but weakly supported clades. Parsimony analysis of the combined data clearly identified three main clades in Coclogyninae. Whereas Coelogyninae are monophyletic, Coelogyne is polyphyletic, with species failing into at least two well-supported clades. The utility of morphological characters used in previous classifications was explored by reconstructing character state evolution on one of the four molecular trees. Lip base and petal shape were homoplasious. whereas ovary indumentum and flower number were congruent with well-supported groups. The implications of our results for the classification of Coelogyne are discussed. and a reorganization of the genus by including Neogyna and Pholidota and removing several species is proposed.