MULTIPLE PEPTIDE IMMUNOREACTIVITIES IN THE NERVOUS-SYSTEM OF AESCHNA-CYANEA (INSECTA, ODONATA) - AN IMMUNOHISTOCHEMICAL STUDY USING ANTISERA TO CHOLECYSTOKININ OCTAPEPTIDE, SOMATOLIBERIN, VASOACTIVE-INTESTINAL-PEPTIDE, MOTILIN AND PROCTOLIN
MULTIPLE PEPTIDE IMMUNOREACTIVITIES IN THE NERVOUS-SYSTEM OF AESCHNA-CYANEA (INSECTA, ODONATA) - AN IMMUNOHISTOCHEMICAL STUDY USING ANTISERA TO CHOLECYSTOKININ OCTAPEPTIDE, SOMATOLIBERIN, VASOACTIVE-INTESTINAL-PEPTIDE, MOTILIN AND PROCTOLIN
复制标题
DOI：
10.1007/bf00315984
复制
发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
TRAMU, G
中科院分区：
文献类型：
--
作者：
ANDRIES, JC;BELEMTOUGRI, G;TRAMU, G
本文用间接免疫过氧化物酶法，对蓝山羊（Aeschna cyanea）的脑、食管下神经节和心体进行了免疫反应，结果表明，蓝山羊的脑、食管下神经节和心体对直肠胆碱抗血清和几种哺乳动物肽类抗血清（包括未硫酸化的八肽胆囊收缩素）均呈免疫反应（CCK-8 NS）（Andries等，1989）、血管活性肠肽（VIP）、人生长激素释放因子（hGRF）（Andries等，1984）和胃动素抗血清。已对苦味酸多聚甲醛溶液或布因奥朗德升华溶液中固定的材料进行了免疫组织化学研究。将抗血清应用于交替切片上，或根据Tramu等人（1978）的洗脱-再染色方法，将抗血清一个接一个地应用于同一切片上。多种肽免疫反应性出现在大脑和食管下神经节表达。hGRF和VIP抗血清反应的细胞也显示胃泌素/CCK样免疫反应性，其中一些也被胃动素抗血清检测到。此外，CCK-8 NS和胃动素抗血清免疫阳性的细胞不显示hGRF或VIP免疫反应。最后，两对原脑细胞出现CCK-8 NS和proctolin抗血清的免疫反应。因此，目前的观察结果支持了我们先前提出的观点（Andries et al. 1989），即CCK样细胞群是异质性的。
By use of the indirect immunoperoxidase method, the brain, the suboesophageal ganglion and the corpora cardiaca of the dragonfly Aeschna cyanea have been shown to be immunoreactive to proctolin antiserum and to several mammalian peptide antisera including unsulfated cholecystokinin octapeptide (CCK-8 NS) (Andries et al. 1989), vasoactive intestinal peptide (VIP), human somatoliberin (hGRF) (Andries et al. 1984) and motilin antisera. Immunohistochemical studies have been performed on material fixed in a solution of picric-acid paraformaldehyde or in Bouin Hollande's sublimate solution. Antisera were applied on alternate sections or, according to the elution-restaining method of Tramu et al. (1978), one after another on the same section. Multiple peptide immunoreactivities appear expressed in the brain and the suboesophageal ganglion. Cells reactive to both hGRF and VIP antisera show also gastrin/CCK-like immunoreactivity and some of them are also detected by motilin antiserum. Besides, some cells immunopositive to CCK-8 NS and motilin antisera do not show hGRF or VIP immunoreactivity. At last, two pairs of protocerebral cells appear immunoreactive to both CCK-8 NS and proctolin antisera. Therefore, the present observations support our previously developed idea (Andries et al. 1989) that the population of CCK-like cells is heterogenous.