DEVELOPMENT OF GLYCINERGIC CELLS AND PUNCTA IN NUCLEI OF THE SUPERIOR OLIVARY COMPLEX OF THE POSTNATAL FERRET
DEVELOPMENT OF GLYCINERGIC CELLS AND PUNCTA IN NUCLEI OF THE SUPERIOR OLIVARY COMPLEX OF THE POSTNATAL FERRET
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DOI：
10.1002/cne.903540313
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发表时间：
1995-04-10
影响因子：
2.5
通讯作者：
BRUNSOBECHTOLD, JK
中科院分区：
文献类型：
--
作者：
HENKEL, CK;BRUNSOBECHTOLD, JK
研究了成年雪貂上级橄榄中甘氨酸免疫阳性细胞和轴突末端的分布。与其他物种一样，成年雪貂上橄榄中最显着的甘氨酸免疫阳性细胞群是梯形体的内侧核。在大多数橄榄周区域，包括外侧和腹侧梯形体核，存在其他深色免疫染色细胞，尽管更分散。在外侧上橄榄核中，甘氨酸免疫阳性细胞与免疫阴性细胞混合。在下丘单侧注射氚化甘氨酸的情况下，对同侧外侧上橄榄核中的类似细胞群进行逆行标记。甘氨酸免疫阳性斑点广泛分布于大部分橄榄周区的神经毡中，其中橄榄周背内侧区以及梯形体的腹侧和外侧核团密集聚集。在外侧和内侧上橄榄核中，免疫阳性斑点分布在特征性周晕中的主细胞周围。在出生后雪貂中，在出生后第 7 天首次观察到免疫阳性细胞体，其分布区域与成人的区域相当，但外侧上橄榄核中的免疫阳性细胞直到出生后 28 天左右才出现。出生后主核和橄榄周核中的神经毡中存在弥漫性染色第7天。在出生后第三周，神经毡中的免疫染色开始呈现出更颗粒状的外观，并且在出生后第35天可以看到免疫阳性的点。在外侧和内侧上橄榄核中，神经毡中免疫染色最早的分布不均匀，分别在高频、内侧和腹侧区域更大。随着免疫染色过程和末端出现在细胞核的大部分区域，这些区域的密度梯度在出生后两周内逐渐消除。 (C) 1995 Wiley-Liss, Inc.
The distribution of glycine-immunopositive cells and axonal endings was studied in the adult and early postnatal ferret superior olive. As in other species, the most prominent glycine-immunopositive cell group in the adult ferret superior olive was the medial nucleus of the trapezoid body. Other darkly immunostained cells were present, although more scattered, in most periolivary regions, including the lateral and ventral trapezoid body nuclei. In the lateral superior olivary nuclei, glycine-immunopositive cells were intermingled with immunonegative cells. A comparable population of cells in the ipsilateral lateral superior olivary nucleus was retrogradely labeled in cases with unilateral injections of tritiated glycine in the inferior colliculus. Glycine-immunopositive puncta were widely distributed in the neuropil in most periolivary regions, including dense accumulations in the dorsomedial periolivary region and ventral and lateral nuclei of the trapezoid body. In the lateral and medial superior olivary nuclei, immunopositive puncta were distributed around the principal cells in characteristic perisomatic halos.In postnatal ferrets, immunopositive cell bodies were first observed by postnatal day 7 and were distributed in regions comparable to regions in the adult, with the exception that immunopositive cells in the lateral superior olivary nucleus did not appear until about postnatal day 28. There was diffuse staining in the neuropil in principal and periolivary nuclei by postnatal day 7. During the third postnatal week, the immunostaining in the neuropil began to take on a more granular appearance and immunopositive puncta could be seen by postnatal day 35. In the lateral and medial superior olivary nuclei, the earliest distribution of immunostaining in the neuropil was nonuniform, being greater in the high-frequency, medial, and ventral regions, respectively. The density gradient in these areas was gradually eliminated over the next 2 postnatal weeks as immunostained processes and endings appeared over greater portions Of the nuclei. (C) 1995 Wiley-Liss, Inc.