Altered gene expression in lymphoblastoid cell lines after subculture.
Altered gene expression in lymphoblastoid cell lines after subculture.
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传代培养后淋巴母细胞系中基因表达发生改变。
DOI：
10.1007/s11626-018-0267-1
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Kishimoto T.
中科院分区：
文献类型：
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作者：
Toritsuka M;Makinodan M;Yamauchi T;Yamashita Y;Ikawa D;Komori T;Kimoto S;Hamano-Iwasa K;Matsuzaki H;Kishimoto T.
类淋巴母细胞系（LCL）是几乎永生化的B淋巴细胞，其用作基因表达分析研究的长期人类细胞供应。然而，关于LCL细胞特征的稳定性的研究很少。为了解决这个问题，我们测量了不同传代次数的LCL中的基因表达，并观察到基因表达在10次传代内发生了实质性变化。特别是，甘油醛-3-磷酸脱氢酶（GAPDH），一个众所周知的管家基因的表达，在传代培养过程中变化很大，因此，使用GAPDH作为内部控制可能是不合适的。总之，本研究强调了在测定LCL中基因表达时需要谨慎。
Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.