Real-time Monitoring of Discrete Synaptic Release Events and Excitatory Potentials within Self-reconstructed Neuromuscular Junctions
Real-time Monitoring of Discrete Synaptic Release Events and Excitatory Potentials within Self-reconstructed Neuromuscular Junctions
复制标题
实时监测自我重建神经肌肉接头内的离散突触释放事件和兴奋电位（热点论文）
DOI：
10.1002/anie.201503801
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发表时间：
2015-08-03
影响因子：
16.6
通讯作者：
Huang, Wei-Hua
中科院分区：
文献类型：
--
作者：
Li, Yu-Tao;Zhang, Shu-Hui;Huang, Wei-Hua
化学突触传递是大脑功能的核心。在这方面，实时监测神经元通信过程中的化学突触传递仍然是一个巨大的挑战。在这项工作中，活体定向神经网络之间的上级颈神经节（SCG）神经元和其效应平滑肌细胞（SMC）组装在一个微流控装置。这允许使用碳纤维纳米电极对功能性SCG-SMC突触内的单个神经递质释放事件进行安培检测，以及使用玻璃纳米移液管电极记录突触后电位。高囊泡释放活动基本上涉及由闪烁融合孔产生的复杂事件，如基于模拟定量建立的。这项工作允许第一次监测原位化学突触传递的条件下发现invivo，这可能会产生重要的和新的见解神经元通信的性质。
Chemical synaptic transmission is central to the brain functions. In this regard, real-time monitoring of chemical synaptic transmission during neuronal communication remains a great challenge. In this work, invivo-like oriented neural networks between superior cervical ganglion (SCG) neurons and their effector smooth muscle cells (SMC) were assembled in a microfluidic device. This allowed amperometric detection of individual neurotransmitter release events inside functional SCG-SMC synapse with carbon fiber nanoelectrodes as well as recording of postsynaptic potential using glass nanopipette electrodes. The high vesicular release activities essentially involved complex events arising from flickering fusion pores as quantitatively established based on simulations. This work allowed for the first time monitoring insitu chemical synaptic transmission under conditions close to those found invivo, which may yield important and new insights into the nature of neuronal communications.