POSSIBLE PRECURSOR OF IMMUNOGLOBULIN LIGHT CHAINS
POSSIBLE PRECURSOR OF IMMUNOGLOBULIN LIGHT CHAINS
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DOI：
10.1038/newbio239117a0
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发表时间：
1972-01-01
期刊：
影响因子：
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通讯作者：
MATHEWS, MB
中科院分区：
文献类型：
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作者：
MILSTEIN, C;HARRISON, TM;MATHEWS, MB
免疫球蛋白轻链在异源无细胞系统中由网织红细胞1和Krebs II腹水细胞2合成，在重建的同源系统中由淋巴结3，4和骨髓瘤5，6合成。虽然合成似乎基本上是真实的，但没有证明与真实轻链的绝对同一性，并且观察到一些小的差异。我们在此报告了某些无细胞反应产物与真实轻链之间的明显差异。与其他无细胞反应的产物没有看到这样的差异。我们建议，轻链最初合成为分子量略高的前体，随后转化为真实的产品。
IMMUNOGLOBULIN light chains are synthesized in heterologous cell-free systems from reticulocytes1and Krebs II ascites cells2, and in reconstituted homologous systems from lymph nodes3,4and myeloma tumours5,6. Although the synthesis appeared to be essentially faithful, absolute identity with authentic light chains was not demonstrated and some small differences were observed. We report here a clear discrepancy between the product of certain cell-free reactions and authentic light chain. With the product of other cell-free reactions no such discrepancy is seen. We propose that light chains are initially synthesized as a precursor of slightly higher molecular weight and subsequently converted into the authentic product.