Longimycelium tulufanense gen. nov., sp. nov., a filamentous actinomycete of the family Pseudonocardiaceae.
Longimycelium tulufanense gen. nov., sp. nov., a filamentous actinomycete of the family Pseudonocardiaceae.
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吐鲁番长丝菌属
DOI：
10.1099/ijs.0.044222-0
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发表时间：
2013
影响因子：
2.8
通讯作者：
Li
中科院分区：
文献类型：
--
作者：
Zhan;Tong;J. Ruan;Ying Huang;Li
从吐鲁番盆地（42°64′N 89°26′E）艾甸湖沉积物中分离到一株新型丝状放线菌，命名为TRM 46004(T)。采用多相法对分离物进行了表征。分离物形成丰富的气生菌丝，分枝少，营养菌丝偶有扭曲和盘绕；球形孢子囊：在气生菌丝的短孢子囊末端发育的球形孢子囊，含有一到几个球形孢子。DNA中G+C含量为65.2 mol%。该分离物含有中二氨基戊酸作为诊断二氨基酸，木糖、半乳糖和核糖是主要的全细胞糖。诊断性磷脂为磷脂酰乙醇胺、磷脂酰胆碱和磷脂酰甘油。甲基萘醌类以MK-9（H4）、MK-9（H6）和MK-9（H10）为主。主要脂肪酸为iso-C16: 0和前iso- c17: 0。基于16S rRNA基因序列的系统发育分析表明，菌株TRM 46004(T)在伪心科中形成了一个独特的谱系，与伪心科成员的16S rRNA基因序列相似性为91.7 ~ 96.1%。在此多相研究的基础上，提出了一个新属和新种——龙根菌属（Longimycelium tulufanense gen. nov., sp. nov.）。土鲁法隆殖菌型菌株为TRM 46004(T) (= CGMCC 4.5737（T) = NBRC 107726(T)）。
A novel filamentous actinomycete strain, designated TRM 46004(T), was isolated from sediment of Aiding Lake in Tulufan Basin (42° 64' N 89° 26' E), north-west China. The isolate was characterized using a polyphasic approach. The isolate formed abundant aerial mycelium with few branches and vegetative mycelium, occasionally twisted and coiled; spherical sporangia containing one to several spherical spores developed at the ends of short sporangiophores on aerial mycelium. The G+C content of the DNA was 65.2 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and xylose, galactose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H10). The major fatty acids were iso-C16 : 0 and anteiso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 46004(T) formed a distinct lineage within the family Pseudonocardiaceae and showed 91.7-96.1 % 16S rRNA gene sequence similarity with members of the family Pseudonocardiaceae. On the basis of the evidence from this polyphasic study, a novel genus and species, Longimycelium tulufanense gen. nov., sp. nov., are proposed. The type strain of Longimycelium tulufanense is TRM 46004(T) (= CGMCC 4.5737(T) = NBRC 107726(T)).
DOI：
10.1099/ijs.0.038075-0
发表时间：
2012-03-01
影响因子：
2.8
作者：
Kim, Ok-Sun;Cho, Yong-Joon;Chun, Jongsik
通讯作者：
Chun, Jongsik