Homo- and heteromeric assembly of TRP channel subunits
Homo- and heteromeric assembly of TRP channel subunits
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DOI：
10.1007/s00424-005-1467-6
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发表时间：
2005-10-01
影响因子：
4.5
通讯作者：
Schaefer, M
中科院分区：
文献类型：
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作者：
Schaefer, M
果蝇瞬时受体电位（TRP）通道的哺乳动物同源物是六螺旋阳离子通道超家族中的第二大阳离子通道家族。大多数哺乳动物TRP通道作为同源寡聚体起作用，并且在被各种刺激激活时介导一价或二价阳离子进入。由于天然TRP通道可能是多聚体蛋白质的可能复杂的组成，这是很难比较天然组织中的阳离子电导的明确定义的同源TRP通道复合物在活细胞中。因此，近年来几个研究小组研究了异聚TRP通道组装的可能性。作为这些研究的一个主要结论，大多数异聚TRP通道复合物似乎仅在相对狭窄的系统发生亚家族范围内由亚基组合组成。虽然现在已经清楚地建立了密切相关的TRP通道亚基之间形成异聚体的一般能力，但我们才刚刚开始了解这些异聚体复合物是否具有生理意义。本文综述了哺乳动物TRP通道的TRPC-、TRPV-和TRPM-亚基组成的通道复合物组装的混杂性和特异性。
Mammalian homologues of the Drosophila melanogaster transient receptor potential (TRP) channels are the second largest cation channel family within the superfamily of hexahelical cation channels. Most mammalian TRP channels function as homooligomers and mediate mono- or divalent cation entry upon activation by a variety of stimuli. Because native TRP channels may be multimeric proteins of possibly complex composition, it is difficult to compare cation conductances in native tissues to those of clearly defined homomeric TRP channel complexes in living cells. Therefore, the possibility of heteromeric TRP channel assembly has been investigated in recent years by several groups. As a major conclusion of these studies, most heteromeric TRP channel complexes appear to consist of subunit combinations only within relatively narrow confines of phylogenetic subfamilies. Although the general capability of heteromer formation between closely related TRP channel subunits is now clearly established, we are only beginning to understand whether these heteromeric complexes are of physiological significance. This review summarizes the current knowledge on the promiscuity and specificity of the assembly of channel complexes composed of TRPC-, TRPV- and TRPM-subunits of mammalian TRP channels.