BCL-2 INCREASES MEMORY B-CELL RECRUITMENT BUT DOES NOT PERTURB SELECTION IN GERMINAL-CENTERS
BCL-2 INCREASES MEMORY B-CELL RECRUITMENT BUT DOES NOT PERTURB SELECTION IN GERMINAL-CENTERS
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DOI：
10.1016/s1074-7613(94)80022-7
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发表时间：
1994-12-01
期刊：
影响因子：
32.4
通讯作者：
TARLINTON, DM
中科院分区：
文献类型：
--
作者：
SMITH, KGC;WEISS, U;TARLINTON, DM
为了解决细胞凋亡在体液免疫反应中的作用，我们在 B 淋巴细胞中表达转基因 Bcl-2 的小鼠中检查了充分表征的 T 细胞依赖性 B 细胞反应。体细胞突变体的选择和高亲和力抗体的出现不受组成型 Bcl-2 表达的影响。然而，这种表达确实不成比例地增加了抗原特异性记忆 B 细胞库，表明记忆区室的最终大小可能受到细胞凋亡过程的调节，而细胞凋亡过程又可能受到 Bcl-2 的影响。此外，转基因小鼠表现出早期抗体产生细胞灶的存活时间延长，这表明它们的去除是由细胞凋亡介导的，而细胞凋亡可以被 Bcl-2 阻断。
To address the role of apoptosis in the humoral immune response, we have examined a well-characterized T cell-dependent B cell response in mice expressing transgenic Bcl-2 in their B lymphocytes. The selection of somatic mutants and the appearance of high affinity antibodies was not affected by constitutive Bcl-2 expression. Such expression did, however, disproportionately increase the antigen-specific memory B cell pool, suggesting that the final size of the memory compartment may be regulated by an apoptotic process, which, in turn, can be influenced by Bcl-2. In addition, transgenic mice showed prolonged survival of foci of early antibody-producing cells, suggesting their removal is mediated by apoptosis that can be blocked by Bcl-2.