An interspersed region of the sea urchin genome represented in both maternal poly(A) RNA and embryo nuclear RNA.
An interspersed region of the sea urchin genome represented in both maternal poly(A) RNA and embryo nuclear RNA.
复制标题
海胆基因组的一个散布区域，以母体 Poly(A) RNA 和胚胎核 RNA 为代表。
DOI：
10.1016/0012-1606(82)90086-0
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发表时间：
1982
影响因子：
2.7
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Thomas,TL;Britten,RJ;Davidson,EH
本文报道了紫球海胆基因组17kb区域的序列组成和转录表达。该区域包括一个转录单位，命名为“基因88”，其由典型的低流行率母体多聚（A）RNA表示。主要的母体基因88转录物的长度约为9.5 kb，对编码该转录物的克隆基因组区域的序列组织分析表明，它含有高密度的低和中等流行的重复序列，以及≥2 kb的单拷贝序列。用来自不同个体海胆的DNA进行的基因组印迹显示，存在这种单拷贝区域的几种等位基因形式。转录的单拷贝序列的多态性仅略低于S.紫色基因组基因88的单拷贝序列似乎在16细胞胚胎的多核糖体RNA中至少有三个较小的转录本。后期胚胎的核RNA也含有基因88的转录本。RNA凝胶印迹表明，多聚腺苷酸化基因88转录物也是9.5 kb的长度在囊胚和原肠胚核RNA，这些转录物可能是共线性的主要母体RNA转录物。
The sequence organization and transcriptional expression of a 17-kb region of theStrongylocentrotus purpuratusgenome are described in this report. This region includes a transcription unit, designated “gene 88,” that is represented by a typical low prevalence maternal poly(A) RNA. The predominant maternal gene 88 transcript is about 9.5 kb in length, and analysis of the sequence organization of the cloned genomic region encoding this transcript shows that it contains a high density of low and moderately prevalent repeat sequences, as well as ≥2 kb of single-copy sequence. Genome blots carried out with DNA from different individual sea urchins show that several allelic forms of this single-copy region exist. The transcribed single-copy sequence is only slightly less polymorphic than is the average single-copy sequence of theS. purpuratusgenome. Gene 88 single-copy sequences appear to be represented in at least three smaller transcripts in the polysomal RNA of 16-cell embryos. The nuclear RNA of later embryos also contains gene 88 transcripts. RNA gel blots indicate that polyadenylated gene 88 transcripts are also 9.5 kb in length in blastula and gastrula nuclear RNAs and these transcripts are probably colinear with the major maternal RNA transcript.