Molt stage and cuticle damage influence white spot syndrome virus immersion infection in penaeid shrimp
Molt stage and cuticle damage influence white spot syndrome virus immersion infection in penaeid shrimp
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DOI：
10.1016/j.vetmic.2009.01.018
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发表时间：
2009-06-12
影响因子：
3.3
通讯作者：
Nauwynck, Hans J.
中科院分区：
文献类型：
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作者：
Corteel, Mathias;Dantas-Lima, Joao J.;Nauwynck, Hans J.
据报道，白斑综合征病毒（WSSV）在对虾中通过饲养和浸泡传播。本研究采用肌肉注射和浸泡两种途径，研究了脱皮过程和角质层人工损伤对虾对WSSV易感性的影响。肌肉注射法，分别在脱壳后早期和晚期、脱壳间和脱壳前早期和后期分别给凡纳滨对虾（n = 450）注射10(-2.3)~ 10（2.7）只感染剂量50%终点（SID50）的WSSV；分别地。A， B， C， D1和d2期。结果表明，注射病毒后，不同蜕皮期仔猪的易感性无显著差异（p < 0.05）。水运实验中，将不同蜕皮阶段的虾浸泡在含有10(4)SID50 ml(-1) WSSV的海水中。在第一项研究中，凡纳梅a （n = 125）在细胞培养瓶中孵育，主要在蜕皮后阶段感染WSSV。在第二项研究中，为了防止角质层损伤，将2组南美单胞虫（n = 100）和单胞虫（n = 100）转移到塑料袋中；其中1组在孵育前切除一只多足动物。两种浸泡法诱导损伤使a期感染由0-40%增加到60-100%，b期从0-20%增加到40-60%，c期从0-20%增加到20-60%，而d期感染均为0% （p < 0.05）。本研究证明，虾在蜕皮后浸泡比蜕皮前更容易感染WSSV，而伤口则有利于感染。(C) 2009 Elsevier B.V.版权所有
Transmission of white spot syndrome virus (WSSV) in shrimp has been reported to occur by feeding and immersion. In the present study, the impact of the molt process and artificial lesions in the cuticle on shrimp susceptibility to WSSV was examined using intramuscular and immersion routes.For the intramuscular route, Penaeus (Litopenaeus) vannamei shrimp (n = 450) were injected with 10(-2.3) Up to 10(2.7) shrimp infectious dose 50% end point (SID50) of WSSV in early and late post-molt, inter-molt, early and late pre-molt; resp. A-, B-, C-, D1- and D2-stage. The resulting infection titers demonstrated that no difference (p > 0.05) in susceptibility existed between different molt stages when virus was injected.For the waterborne route, shrimp in different molt stages were immersed in seawater containing 10(4) SID50 ml(-1) of WSSV. In a first study, A vannamei (n = 125) incubated in cell culture flasks, became infected with WSSV mostly in post-molt stages. In a second study, 2 groups of A vannamei (n = 100) and P. monodon (n = 100) were transferred into plastic bags to prevent damage to the cuticle; and in 1 group a pleopod was cut off prior to incubation. Induction of damage increased infection significantly (p < 0.05) in A-stage from 0-40% to 60-100%, in B-stage from 0-20% to 40-60%, in C-stage from 0-20 to 20-60%, while infection was 0% in D-stages with both immersion methods.This study proved that shrimp are more susceptible to WSSV infection via immersion after molting than in the period before molting and wounding facilitates infection. (C) 2009 Elsevier B.V. All rights reserved.