Identification of QTL for seed dormancy from weedy rice and its application to elite rice cultivar 'Ninggeng 4'
Identification of QTL for seed dormancy from weedy rice and its application to elite rice cultivar 'Ninggeng 4'
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杂草稻种子休眠QTL鉴定及其在良种‘宁耕4号’中的应用
DOI：
10.1007/s11032-019-1031-2
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发表时间：
2019
影响因子：
3.1
通讯作者：
Wan Jianmin
中科院分区：
文献类型：
--
作者：
Thanhliem Nguyen;Zhou Chunlei;Zhang Tianyu;Yu Jiangfeng;Miao Rong;Huang Yunshuai;Zhu Xingjie;Song Weihan;Liu Xi;Mou Changling;Lan Jie;Liu Shijia;Tian Yunlu;Zhao Zhigang;Jiang Ling;Wan Jianmin
Seed dormancy is a complex agronomic trait that is not only controlled by genetics but also influenced by environmental conditions. Novel seed dormancy genes could improve rice yield and quality by preventing losses due to pre-harvest sprouting (PHS). Although there have been many reported studies related to seed dormancy in rice, little is known about seed dormancy genes derived from weedy rice. We used a weedy rice line ‘Ludao’ (with strong seed dormancy) as the donor parent and elitejaponicavariety ‘Ninggeng 4’ (with weak seed dormancy) as a recurrent parent for backcrossing. A BC4F2population was developed for gene mapping, and germination percentage was used as the phenotype. Four quantitative trait loci (QTLs),qSdr7-1,qSdr7-2,qSdr7-3, andqSdr7-4, were identified on chromosome 7. Seed dormancy alleles of the QTLs were all derived from ‘Ludao.’ Located between markers RM21103 and RM5672,qSdr7-2had the largest effect and explained 27.2% of the phenotypic variation with a LOD score of 14.5. Based on the closest linked marker for locusqSdr7-2, we developed near-isogenic lines (NILs) with theqSdr7-2allele from ‘Ludao’ in ‘Ninggeng 4’ background. TheqSdr7-2-NILs, which have strong seed dormancy and elite agronomic traits, should be useful as a parent in breeding for PHS resistance.
