Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater
Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater
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DOI：
10.1021/acs.estlett.3c00080
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发表时间：
2023-03-17
影响因子：
10.9
通讯作者：
Wang，Phillip
中科院分区：
文献类型：
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作者：
Smith，Adam L.;Wang，Phillip
抗生素耐药基因（ARG）的废水监测提供了深入了解其服务人口的循环抗生素耐药概况。对废水ARG的持续监测和表征可以帮助识别具有临床重要性的新兴ARG及其在细菌物种和地点之间的传播。在这项研究中，我们在2020年9月至2022年2月期间在洛杉矶县废水中检测到四种移动的粘菌素耐药（mcr）基因变异体（mcr-3.12、mcr-4.3、mcr-5.1和mcr-9.1）。通过对培养的粘菌素耐药细菌分离株和废水质粒进行测序，我们表征了检测到的mcr基因变体的遗传环境，并揭示了我们的数据集和已发表数据之间的保守mcr基因盒。此外，我们发现了两个新的小质粒（约8.29和8.67 kbp）与近identicalmcr-5基因盒两侧的非同源区域。在培养的粘菌素耐药细菌分离株中，我们检测到铜绿气单胞菌中mcr-3.12和蜂房哈夫尼菌中mcr-9.1的存在。我们的研究结果强调了废水监测的实用性，以检测和表征循环ARGs，并帮助发现中间载体沿着他们的传播途径。
Wastewater surveillance of antibiotic resistance genes (ARGs) provides insight into the circulating antibiotic-resistant profile of its serviced population. Continual surveillance and characterization of wastewater ARGs could help identify emerging ARGs of clinical importance and their dissemination across bacterial species and locations. In this study, we detected four mobile colistin-resistant (mcr) gene variants (mcr-3.12,mcr-4.3,mcr-5.1, andmcr-9.1) in Los Angeles County wastewater between September 2020 to February 2022. Through sequencing of cultured colistin-resistant bacterial isolates and wastewater plasmids, we characterized the genetic environments of the detectedmcrgene variants and revealed conservedmcrgene cassettes between our data set and published data. Moreover, we discovered two novel small plasmids (approximately 8.29 and 8.67 kbp) with near identicalmcr-5 gene cassettes flanked by a nonhomologous region. Within cultured colistin-resistant bacterial isolates, we detected the presence ofmcr-3.12 inAeromonas hydrophilaandmcr-9.1 inHafnia alvei. Our findings underscore the utility of wastewater surveillance to detect and characterize circulating ARGs and help uncover intermediate carriers along their dissemination routes.