Validation of two multiplex platforms to quantify circulating markers of inflammation and endothelial injury in severe infection
Validation of two multiplex platforms to quantify circulating markers of inflammation and endothelial injury in severe infection
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DOI：
10.1371/journal.pone.0175130
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发表时间：
2017-04-18
期刊：
影响因子：
3.7
通讯作者：
Kain, Kevin C.
中科院分区：
文献类型：
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作者：
Leligdowicz, Aleksandra;Conroy, Andrea L.;Kain, Kevin C.
生物标志物可以预测结果并实现风险分层。在严重感染中，关注反映器官损伤的病理生理机制的多个标志物可以加强管理和途径导向治疗。关于多重生物标志物平台的性能的数据有限。我们的目标是使用两种多重平台比较严重儿科感染中的内皮和免疫活化生物标志物。来自乌干达金贾地区医院的410名疑似感染儿童的冷冻血浆用于测量内皮细胞（血管生成素-2，sFlt-1，sVCAM-1，sICAM-1）和免疫（IL-6，IP-10，sTNFR-1，CHI 3L 1）激活的生物标志物。使用来自R&D Systems的试剂选择基于使用生物素-链霉亲和素缀合物化学的单克隆抗体夹心免疫测定的两种多重平台（Luminex（R），Ella（TM））。两个平台在完成的容易程度和时间、每次测定的样品数量和动态浓度范围方面存在差异。使用变异系数（CV%）评估的试验内变异性对于Luminex（R）为2.2-3.4，对于Ella TM为1.2-2.9。在两个平台的动态范围内，生物标志物浓度的相关性对于IL-6最好（p = 0.96，p
Biomarkers can prognosticate outcome and enable risk-stratification. In severe infection, focusing on multiple markers reflecting pathophysiological mechanisms of organ injury could enhance management and pathway-directed therapeutics. Limited data exist on the performance of multiplex biomarker platforms. Our goal was to compare endothelial and immune activation biomarkers in severe pediatric infections using two multiplex platforms. Frozen plasma from 410 children presenting to the Jinja Regional Hospital in Uganda with suspected infection was used to measure biomarkers of endothelial (Angiopoietin-2, sFlt-1, sVCAM-1, sICAM-1) and immune (IL-6, IP-10, sTNFR-1, CHI3L1) activation. Two multiplex platforms (Luminex (R), Ella (TM)) based on monoclonal antibody sandwich immunoassays using biotin-streptavidin conjugate chemistry were selected with reagents from R&D Systems. The two platforms differed in ease and time of completion, number of samples per assay, and dynamic concentration range. Intra-assay variability assessed using a coefficient of variation (CV%) was 2.2-3.4 for Luminex (R) and 1.2-2.9 for Ella TM. Correlations for biomarker concentrations within dynamic range of both platforms were best for IL-6 (p = 0.96, p