Extracts of passion fruit peel and seed of Passiflora edulis (Passifloraceae) attenuate oxidative stress in diabetic rats
Extracts of passion fruit peel and seed of Passiflora edulis (Passifloraceae) attenuate oxidative stress in diabetic rats
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DOI：
10.1016/s1875-5364(15)30066-2
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发表时间：
2015-09-01
影响因子：
4.6
通讯作者：
Ahamed, Haja N.
中科院分区：
文献类型：
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作者：
Kandandapani, Salanee;Balaraman, Ashok K.;Ahamed, Haja N.
本研究旨在探讨百香果西番莲（EPE）提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠氧化应激的抗糖尿病作用。将30只成年Wistar大鼠分为5组，每组6只。对照组大鼠腹腔注射柠檬酸缓冲液（pH为4.5）。其余各组大鼠经腹腔注射STZ 45 mg.kg（-1）单次诱导糖尿病。糖尿病动物分别口服EPE 250、500 mg.kg（-1）和格列本脲0.6 mg.kg(-1) 15 d。测定血糖、终器官氧化应激标志物及抗氧化剂。实验结束时进行胰腺组织病理学检查。结果显示，亚急性给药EPE能显著控制糖尿病大鼠的血糖水平（P < 0.001）。此外，EPE提取物通过恢复抗氧化酶，显著提高内脏器官超氧化物歧化酶（SOD）水平，降低过氧化氢酶（CAT）和TBARS水平，对终器官具有保护作用。综上所述，EPE提取物对链脲佐菌素诱导的糖尿病具有抗糖尿病和抗氧化作用。
This study was aimed at evaluating the anti-diabetic potential of passion fruit Passiflora edulis (EPE) extracts in diabetic rats, following Streptozotocin (STZ) induced oxidative stress. Thirty adult Wistar rats were divided into five groups, with six rats in each group. The control rats were injected intraperitoneally with citrate buffer (pH 4.5). The remaining groups of rats were administered single dose of 45 mg.kg(-1) of STZ by intraperitoneal route to induce diabetes. The diabetic animals were treated with 250 and 500 mg.kg(-1) of EPE and glibenclamide 0.6 mg.kg(-1) for fifteen days by oral route. Blood glucose, end organ oxidative stress marker, and anti-oxidants were assayed. Further, histopathological investigation of pancreas was studied at the end of the experimentation. The results revealed that subacute administration of EPE significantly (P < 0.001) controlled the blood glucose level in the diabetic rats. In addition, EPE extract protected the end organs by restoring the anti-oxidants enzyme, significantly increasing super oxide dismutase level (SOD) and decreasing catalase (CAT) and TBARS level in visceral organs. In conclusion, that EPE extracts showed anti-diabetic and anti-oxidant potential against streptozotocin-induced diabetes.