OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories
OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories
复制标题
DOI：
10.1128/aac.00818-17
复制
发表时间：
2017-09-01
影响因子：
4.9
通讯作者：
Dortet, Laurent
中科院分区：
文献类型：
--
作者：
Hoyos-Mallecot, Yannick;Naas, Thierry;Dortet, Laurent
OXA-244是OXA-48的单点突变衍生物，显示碳青霉烯酶活性降低。在这里，我们报告了7株产生oxa -244的大肠杆菌的微生物学特征。只有一个分离株在ChromID Carba Smart培养基（bioMerieux）上生长，但7个分离株中有6个在ChromID扩展光谱- β -内酰胺酶（ESBL）培养基（bioMerieux）上生长，因为它们共同产生ESBL和/或质粒编码的头孢菌素酶。通过Carba NP测试、Rapidec Carba NP测试（bioMerieux）、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）水解测试（Bruker）和OXA-48 K-SeT测试（Coris BioConcept），分别检测到57.1%、71.4%、71.4%和100%的大肠杆菌分离物产生碳青霉烯酶。我们的研究结果表明，产生oxa -244的大肠杆菌分离株难以检测到，这可能导致其沉默传播。
OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMerieux), but six of the seven isolates grew on ChromID extended-spectrum-beta-lactamase (ESBL) medium (bioMerieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the Rapidec Carba NP test (bioMerieux), a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) hydrolysis assay (Bruker), and the OXA-48 K-SeT assay (Coris BioConcept), respectively. Our results indicate that OXA-244-producing E. coli isolates are difficult to detect, which may lead to their silent spread.