Coordinating structural and functional synapse development: Postsynaptic p21-activated kinase independently specifies glutamate receptor abundance and postsynaptic morphology
Coordinating structural and functional synapse development: Postsynaptic p21-activated kinase independently specifies glutamate receptor abundance and postsynaptic morphology
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DOI：
10.1523/jneurosci.1538-04.2004
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发表时间：
2004-08-04
影响因子：
5.3
通讯作者：
Davis, GW
中科院分区：
文献类型：
--
作者：
Albin, SD;Davis, GW
在这里，我们表明，突触后p21激活激酶（Pak）信号分歧成两个遗传上可分离的途径在果蝇神经肌肉接头。一种途径控制谷氨酸受体丰度。该途径内的Pak信号传导由与衔接蛋白Dreadlocks（Dock）的所需相互作用指定。我们证明，码头是本地化的突触通过Src同源2介导的蛋白质相互作用。Dock对于Pak定位不是必需的，但对于限制Pak信号传导以控制谷氨酸受体丰度是必需的。Pak激酶信号传导的第二个遗传上可分离的功能通过调节突触盘-大来控制肌膜特化。在这条通路中，Dock是一个双胞胎。我们提出了一个模型，其中不同的Pak信号能够协调两个不同的功能，突触后成熟，受体丰度和肌膜专业化。
Here, we show that postsynaptic p21-activated kinase (Pak) signaling diverges into two genetically separable pathways at the Drosophila neuromuscular junction. One pathway controls glutamate receptor abundance. Pak signaling within this pathway is specified by a required interaction with the adaptor protein Dreadlocks ( Dock). We demonstrate that Dock is localized to the synapse via an Src homology 2-mediated protein interaction. Dock is not necessary for Pak localization but is necessary to restrict Pak signaling to control glutamate receptor abundance. A second genetically separable function of Pak kinase signaling controls muscle membrane specialization through the regulation of synaptic Discs-large. In this pathway, Dock is dispensable. We present a model in which divergent Pak signaling is able to coordinate two different features of postsynaptic maturation, receptor abundance, and muscle membrane specialization.