Allele-specific MVR-PCR analysis at minisatellite D1S8.
Allele-specific MVR-PCR analysis at minisatellite D1S8.
复制标题
小卫星 D1S8 的等位基因特异性 MVR-PCR 分析。
DOI：
10.1093/hmg/2.5.513
复制
发表时间：
1993
影响因子：
3.5
通讯作者：
A. Jeffreys
中科院分区：
文献类型：
--
作者：
D. Monckton;K. Tamaki;A. MacLeod;D. L. Neil;A. Jeffreys
聚合酶链反应（MVR-PCR）的小卫星变异重复图谱为DNA分型提供了一种数字化方法，在法医学中具有巨大的潜在用途，并且通过绘制单个等位基因图谱，探索小卫星上的等位基因变异和突变过程。单个等位基因的MVR单倍型既可以从物理分离的等位基因中确定，也可以通过对两个等位基因同时产生的数字二倍体代码进行系谱分析来确定。我们现在表明，使用等位基因特异性PCR引物，单个等位基因可以从总基因组DNA中快速定位到小卫星两侧DNA的多态性位点。这种方法也可用于解剖混合DNA样本，如在法医DNA分析中经常遇到的那些。
Minisatellite variant repeat mapping by the polymerase chain reaction (MVR-PCR) provides a digital approach to DNA typing of great potential use both in forensic medicine and, by mapping single alleles, for exploring allelic variability and mutation processes at minisatellites. The MVR haplotypes of single alleles can be determined either from physically separated alleles or by pedigree analysis of digital diploid codes generated from both alleles simultaneously. We now show that single alleles can be rapidly mapped from total genomic DNA using allele-specific PCR primers directed to polymorphic sites in the DNA flanking the minisatellite. This approach can also be used to dissect mixed DNA samples such as those often encountered in forensic DNA analysis.