Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit.
Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit.
复制标题
哺乳动物细胞有丝分裂中的蛋白质磷酸酶1型：染色体定位和参与有丝分裂出口。
DOI：
10.1083/jcb.116.6.1421
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发表时间：
1992-03
影响因子：
7.8
通讯作者：
Lamb, N J
中科院分区：
文献类型：
--
作者：
Fernandez, A;Brautigan, D L;Lamb, N J
我们研究了蛋白磷酸酶1（PP-1）在哺乳动物细胞有丝分裂中的作用。使用抗PP-1抗体的免疫荧光显示，PP-1，这是主要定位在G1和S期细胞的细胞质中，积累在细胞核中的G2期和强烈的共定位与个别染色体在有丝分裂。在G2/M细胞中核PP-1的这种增加通过亚细胞组分的免疫印迹证实。在分裂前显微注射中和性抗PP-1抗体可阻断分裂中期的细胞，而在分裂后期B细胞的一极注射PP-1则可加速胞质分裂和注射细胞的增殖。这些结果揭示了一个特定的细胞周期依赖性的再分配PP-1和它的参与逆转p34 cdc 2诱导的效应后中期有丝分裂在哺乳动物细胞。
We have examined the role of protein phosphatase type 1 (PP-1) in mammalian cell mitosis. Immunofluorescence using anti-PP-1 antibodies revealed that PP-1, which is mainly localized in the cytoplasm of G1 and S phase cells, accumulates in the nucleus during G2 phase and intensely colocalizes with individual chromosomes at mitosis. This increase in nuclear PP-1 in G2/M cells was confirmed by immunoblotting on subcellular fractions. Microinjection of neutralizing anti-PP-1 antibodies before division blocked cells at metaphase, whereas injection of PP-1 in one pole of an anaphase B cell accelerated cytokinesis and the reflattening of the injected cell. These results reveal a specific cell cycle-dependent redistribution of PP-1 and its involvement in reversing p34cdc2-induced effects after mid-mitosis in mammalian cells.