Myofibroblasts are associated with the progression of scirrhous gastric carcinoma
Myofibroblasts are associated with the progression of scirrhous gastric carcinoma
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DOI：
10.3892/etm_00000086
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发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Hirakawa, Kosei
中科院分区：
文献类型：
--
作者：
Fuyuhiro, Yuhiko;Yashiro, Masakazu;Hirakawa, Kosei
成纤维细胞，特别是肌成纤维细胞，影响体外癌细胞的恶性进展。然而，到目前为止，很少有报告已经解决了肌成纤维细胞在胃癌微环境中的临床意义。本研究旨在探讨肌成纤维细胞在胃癌中的表达与临床病理特征的关系。用抗α-平滑肌肌动蛋白和波形蛋白的抗体对265例胃切除术后的原发性胃肿瘤进行染色。波形蛋白阳性的基质细胞被认为是成纤维细胞。肌成纤维细胞定义为α-平滑肌染色阳性的成纤维细胞。当肌成纤维细胞占癌间质中成纤维细胞的25%以上时，建立肌成纤维细胞阳性胃癌。265例胃癌中肌成纤维细胞表达阳性者92例（35%）。肌成纤维细胞的表达在进展期胃癌中（146例中的76例）明显高于早期胃癌（119例中的16例）（p < 0.001）。肌成纤维细胞的表达与硬癌（p < 0.001）、淋巴结转移（p <0.001）、淋巴管浸润（p <0.001）和腹膜转移（p=0.005）相关。肌成纤维细胞表达阳性的肿瘤患者的预后明显较差（p < 0.001），而多变量分析显示肌成纤维细胞表达不是独立的预后因素。这些结果表明，肌成纤维细胞与硬癌。因此，肌成纤维细胞的过度表达可能是一个有用的胃癌预后指标。
Fibroblasts, particularly myofibroblasts, affect the malignant progression of cancer cells in vitro. However, to date few reports have addressed the clinical significance of myofibroblasts in the gastric cancer microenvironment. This study examined the correlation between myofibroblast expression and clinicopathological features in gastric carcinoma. A total of 265 primary gastric tumors resected by gastrectomy were stained with antibodies against alpha-smooth muscle actin and vimentin. Stromal cells positive for vimentin were considered to be fibroblasts. Myofibroblasts were defined as fibroblasts positive for alpha-smooth muscle staining. Myofibroblast-positive gastric carcinoma was established when myofibroblasts accounted for more than 25% of fibroblasts in the cancer stroma. Myofibroblast expression was positive in 92 (35%) of the 265 gastric carcinomas. Myofibroblast expression showed a significantly (p < 0.001) high frequency in advanced gastric cancers (76 of 146), in comparison to the early stage cancers (16 of 119). Taken together, there was a statistically significant correlation between myofibroblast expression and scirrhous type gastric cancer (p < 0.001), lymph node metastasis (p < 0.001), lymphatic invasion (p < 0.001) and peritoneal dissemination (p=0.005). The prognosis of patients with tumors positive for myofibroblast expression was significantly (p < 0.001) worse, while a multivariate analysis revealed that myofibroblast expression was not an independent prognostic factor. These findings suggest that myofibroblasts are associated with scirrhous gastric cancer. Overexpression of myofibroblasts may therefore be a useful prognostic indicator of gastric carcinoma.