EFFECT OF LONG-CHAIN FATTY-ACIDS ON BACTERIAL RESPIRATION AND AMINO-ACID UPTAKE
EFFECT OF LONG-CHAIN FATTY-ACIDS ON BACTERIAL RESPIRATION AND AMINO-ACID UPTAKE
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DOI：
10.1111/j.1365-2672.1973.tb04151.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
MILLER, TB
中科院分区：
文献类型：
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作者：
GALBRAITH, H;MILLER, TB
长链脂肪酸在杀菌和原生质体溶解浓度下刺激革兰氏阳性菌的氧摄取，并在更高水平下产生抑制作用。单个酸之间的活性顺序和逆转剂对呼吸活性的影响对应于产生杀菌活性的那些。原生质体比全细胞更容易受到抑制。在高脂肪酸浓度下，革兰氏阴性菌受到有限程度的抑制，但原生质球高度敏感。在亚杀菌水平下，脂肪酸在需氧和厌氧条件下均抑制氨基酸摄取。金属离子可逆转此效应，并反映了二硝基酚和叠氮化钠的活性。微生物对抑制的敏感性与对其他抗菌作用的敏感性处于同一顺序。的脂肪酸的作用的可能模式进行了讨论，在干扰细菌细胞内的能量代谢。
Summary.Long chain fatty acids stimulated oxygen uptake by Gram positive bacteria at bactericidal and protoplast lytic concentrations and produced inhibition at higher levels. The order of activity between individual acids and effects of reversal agents on respiratory activity corresponded to those which produced bactericidal activity. Protoplasts were more susceptible to inhibition than whole cells. Gram negative bacteria were inhibited to a limited extent at high fatty acid concentrations, but spheroplasts were highly sensitive. Fatty acids inhibited amino acid uptake both aerobically and anaerobically at sub‐bactericidal levels. The effects were reversed by metal cations, and reflected the activity of dinitrophenol and sodium azide. The susceptibility of organisms to inhibition was of the same order as the sensitivity to other antibacterial effects. The probable mode of action of the fatty acids is discussed in terms of the interference with energy metabolism within the bacterial cell.