Nitric oxide reduces seed dormancy in Arabidopsis
Nitric oxide reduces seed dormancy in Arabidopsis
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DOI：
10.1093/jxb/erj060
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发表时间：
2006-02-01
影响因子：
6.9
通讯作者：
Jones, RL
中科院分区：
文献类型：
--
作者：
Bethke, PC;Libourel, IGL;Jones, RL
休眠是许多成熟种子的一种特性，在过去的世纪里，实验已经确定了许多减少种子休眠的化学处理。含氮化合物，包括硝酸盐，亚硝酸盐和氰化物打破种子休眠的范围内的物种。这里描述的实验进行，以进一步了解这些和其他化合物，如一氧化氮（NO）供体硝普钠（SNP），带来减少种子休眠的拟南芥的机制。设计了一种简单的方法用于通过气相应用SNP光解产物。使用这种方法，表明SNP以及铁氰化钾（Fe（III）CN）和亚铁氰化钾（Fe（II）CN）通过产生氰化物（CN）来降低拟南芥种子的休眠。本文研究了氰化钾（KCN）对拟南芥种子休眠的影响，证实氰化物蒸气足以打破拟南芥种子休眠。硝酸盐和亚硝酸盐也降低了拟南芥种子的休眠，并导致了大量的发芽率。NO清除剂c-PTIO可有效抑制CN、亚硝酸盐和硝酸盐对休眠的影响。利用纯化的NO气体证实了NO在降低种子休眠中的作用，并提出了一个解释含氮化合物如何打破拟南芥种子休眠的模型。
Dormancy is a property of many mature seeds, and experimentation over the past century has identified numerous chemical treatments that will reduce seed dormancy. Nitrogen-containing compounds including nitrate, nitrite, and cyanide break seed dormancy in a range of species. Experiments are described here that were carried out to further our understanding of the mechanism whereby these and other compounds, such as the nitric oxide (NO) donor sodium nitroprusside (SNP), bring about a reduction in seed dormancy of Arabidopsis thaliana. A simple method was devised for applying the products of SNP photolysis through the gas phase. Using this approach it was shown that SNP, as well as potassium ferricyanide (Fe(III)CN) and potassium ferrocyanide (Fe(II)CN), reduced dormancy of Arabidopsis seeds by generating cyanide (CN). The effects of potassium cyanide (KCN) on dormant seeds were tested and it was confirmed that cyanide vapours were sufficient to break Arabidopsis seed dormancy. Nitrate and nitrite also reduced Arabidopsis seed dormancy and resulted in substantial rates of germination. The effects of CN, nitrite, and nitrate on dormancy were prevented by the NO scavenger c-PTIO. It was confirmed that NO plays a role in reducing seed dormancy by using purified NO gas, and a model to explain how nitrogen-containing compounds may break dormancy in Arabidopsis is presented.