Herpesviral deoxythymidine kinases contain a site analogous to the phosphoryl-binding arginine-rich region of porcine adenylate kinase; comparison of secondary structure predictions and conservation.
Herpesviral deoxythymidine kinases contain a site analogous to the phosphoryl-binding arginine-rich region of porcine adenylate kinase; comparison of secondary structure predictions and conservation.
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疱疹病毒脱氧胸苷激酶含有与猪腺苷酸激酶的磷酰结合富含精氨酸区域类似的位点；
DOI：
10.1099/0022-1317-71-12-2979
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Gentry,GA
中科院分区：
文献类型：
--
作者：
Balasubramaniam,NK;Veerisetty,V;Gentry,GA
12疱疹病毒脱氧胸苷激酶的序列相似性区域进行了检查，通过多重比对。观察到6个高度保守的位点。位点1对应于形成猪腺苷酸激酶（PAK）中ATP结合口袋的一部分的富含甘氨酸的环，位点5对应于PAK中的一个区域，位于裂的一个叶上，其含有结合底物磷酰基的精氨酸残基。由基序-DRH-组成的位点3被认为参与胸腺嘧啶/脱氧胸苷识别;附近的位点4可能也参与该功能。2号和6号站点的功能尚未确定。通过Gamier方法进行二级结构预测，并对多重比对中的每个位置取平均值。预测的所有六个位点的结构通常是一个短的柔性区域（转弯或线圈）或附近的网站，两侧的刚性结构（螺旋或片）。
Twelve herpesviral deoxythymidine kinases were examined for regions of sequence similarity by multiple alignment. Six highly conserved sites were observed. Site 1 corresponded to a glycine-rich loop that forms part of the ATP-binding pocket in porcine adenylate kinase (PAK), and site 5 corresponded to a region in PAK, located on one lobe of the cleft, that contains arginine residues that bind substrate phosphoryl groups. Site 3, consisting of the motif -DRH-, is thought to be involved in thymine/deoxythymidine recognition; site 4, which is nearby, probably participates in this function as well. The functions of sites 2 and 6 have not been identified. Secondary structure predictions were made by the Gamier method and averaged for each position in the multiple alignment. The structure predicted for all six sites was typically a short flexible region (turn or coil) at or adjacent to the site, flanked by rigid structures (helix or sheet) on either side.