Treadmill Exercise as a Preventive Measure Against Age-Related Anxiety and Social Behavioral Disorders in Rats: When Is It Worth Starting?
Treadmill Exercise as a Preventive Measure Against Age-Related Anxiety and Social Behavioral Disorders in Rats: When Is It Worth Starting?
复制标题
DOI：
10.5535/arm.22105
复制
发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Tanaka J
中科院分区：
文献类型：
--
作者：
Taguchi S;Choudhury ME;Mikami K;Utsunomiya R;Yano H;Tanaka J
确定开始定期锻炼的适当时间点，以减少与年龄相关的焦虑和社交行为受损。本研究将8周龄雄性Wistar大鼠分为3组：无运动组（NoEX）、短期运动组（S-Ex）和长期运动组（L-Ex）。s - ex组大鼠12月龄开始跑步机运动，L-Ex组大鼠2月龄开始跑步机运动。运动大鼠每周被迫在跑步机上行走三次，前两周以10米/分钟的速度行走，间隔1至2天，每次10分钟，直到17个月大，之后以8米/分钟的速度行走。在19个月大时，进行行为测试以评估运动对年龄引起的行为变化的影响，并进行定量聚合酶链反应以揭示行为变化背后的机制。焦虑样行为通过长期锻炼得到改善。此外，S-Ex组和L-Ex组的大鼠表现出更好的社会行为，对有趣的物体的好奇心也增加了。qPCR数据显示，跑步机运动抑制了老年大鼠前额叶皮层中立即早期基因的表达。这项研究表明，长期运动抑制了早期反应基因，从而增强了对压力的抵抗力，减少了与焦虑相关的行为，改善了社会行为。这些发现强调需要考虑适当的时间开始锻炼，以防止压力引起的焦虑相关行为。
To determine the appropriate time points to start regular exercise which could reduce age-related anxiety and impaired social behavior. For this study, 8-week-old male Wistar rats were divided into three groups: no exercise (NoEX), short-term exercise (S-Ex), and long-term exercise (L-Ex) groups. S-Ex-group rats started treadmill exercise at 12 months of age, while L-Ex rats started from at 2 months of age. Exercise rats were forced to walk on the treadmill three times per week, with 1- to 2-day intervals for 10 minutes during the first 2 weeks, at 10 m/min until 17 months of age, and at 8 m/min thereafter. At 19 months of age, behavioral tests were performed to assess the effects of exercise on age-induced behavioral change as well as quantitative polymerase chain reaction were done to uncover the mechanism behind the behavioral changes. Anxiety-like behavior was improved by long-term exercise. Additionally, rats belonging to the S-Ex and L-Ex groups showed improved social behavior and increased curiosity about interesting objects. The qPCR data showed that treadmill exercise suppressed the expression of immediate-early genes in the prefrontal cortex of the aged rats. This study suggests that long-term exercise represses early response genes, and in this way, it increases resistance to stress, diminishes anxiety-related behavior, and improves social behavior. These findings underscore the need to consider appropriate time to start exercise to prevent stress induced anxiety related behavior.