Cytotoxicity and biological effects of functional nanomaterials delivered to various cell lines
Cytotoxicity and biological effects of functional nanomaterials delivered to various cell lines
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DOI：
10.1002/jat.1475
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Biris, Alexandru S.
中科院分区：
文献类型：
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作者：
Mahmood, Meena;Casciano, Daniel A.;Biris, Alexandru S.
将包括金、银纳米颗粒和单壁碳纳米管在内的功能纳米材料以不同浓度递送至两种细胞系（MLO-Y4 骨细胞和 HeLa 宫颈癌细胞）。研究发现细胞在相对较短的时间内吸收了纳米材料，这一过程显着影响了细胞的形状和大小。研究发现，由于这些纳米材料的输送而导致的细胞死亡百分比对于碳纳米管来说是最高的，并且随着这些纳米结构的浓度而逐渐增加。此外，当将纳米材料与常用化疗药物如依托泊苷或地塞米松联合递送至细胞时，与仅递送纳米材料或化疗药物的情况相比，死亡细胞的数量显着增加(100-300%)。实验结果得到了Caspase 3研究的证实，表明本研究中使用的纳米材料与细胞的蛋白质结构之间存在很强的相互作用，这使得细胞凋亡剂能够更有效地发挥作用。这些发现可能成为由化疗药物和纳米材料组成的新型癌症疗法的基础。版权所有 (C) 2009 John Wiley & Sons, Ltd.
Functional nanomaterials that included gold, silver nanoparticles and single wall carbon nanotubes were delivered to two cell lines (MLO-Y4 osteocytic cells and HeLa cervical cancer cells) in various concentrations. The cells were found to uptake the nanomaterials in a relatively short time, a process that significantly affected the shape and the size of the cells. The percentage of cellular death, due to the delivery of these nanomaterials, was found to be the highest for carbon nanotubes and increased gradually with the concentration of these nanostructures. Moreover, when the nanomaterials were delivered to the cells combined with commonly used chemotherapeutic agents such as etoposide or dexamethasone, the number of the cells that died increased significantly (100-300%) as compared with the case when only the nanomaterials or the chemotherapeutic agents were delivered. The experimental results were confirmed by Caspase 3 studies, indicating a strong interaction between the nanomaterials used in this study and the protein structure of the cells, which allowed a more effective action of the apoptotic agents. These findings could be the foundation of a new class of cancer therapies that are composed of both chemotherapeutic agents and nanomaterials. Copyright (C) 2009 John Wiley & Sons, Ltd.