Natural killer and natural killer-T cells in psoriasis
Natural killer and natural killer-T cells in psoriasis
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DOI：
10.1007/s00403-002-0349-4
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发表时间：
2002-11-01
影响因子：
3
通讯作者：
Griffiths, CEM
中科院分区：
文献类型：
--
作者：
Cameron, AL;Kirby, B;Griffiths, CEM
银屑病的特征是真皮和表皮浸润，主要由CD 4（+）和CD 8（+）T细胞组成。这些细胞以抗原依赖的方式表现，这表明银屑病可能是一种T细胞介导的自身免疫性疾病。银屑病与公认的自身免疫性疾病如I型糖尿病和多发性硬化症具有某些免疫学特征，在这两种疾病中，假定自然杀伤（NK）细胞和自然杀伤样T（NK-T）细胞具有致病作用。然而，很少有研究评估NK和NK-T细胞在银屑病中的作用。我们试图确定NK和NK-T细胞是否存在于银屑病中。从10名慢性斑块型银屑病患者的银屑病斑块的活动边缘和距离受累皮肤至少5 cm的未受累皮肤进行皮肤活检。来自四个正常受试者的皮肤用作对照。使用免疫过氧化物酶技术，使用T细胞标志物CD 2、CD 3、CD 4和CD 8;皮肤白细胞相关抗原; NK细胞标志物CD 16、CD 56、CD 57、CD 94和CD 158 a;以及NK-T细胞标志物CD 161的抗体对冷冻切片进行染色。皮损中表达T细胞标志、NK细胞标志CD 16、CD 57、CD 94、CD 158 a和NK-T细胞标志CD 161的细胞明显多于正常皮肤和非皮损皮肤（P
Psoriasis is characterized by a dermal and epidermal infiltrate comprised predominantly of CD4(+) and CD8(+) T cells, respectively. These cells behave in an antigen-dependent manner, which suggests that psoriasis may be a T-cell-mediated autoimmune disease. Psoriasis shares certain immunological features with recognized autoimmune conditions such as type I diabetes mellitus and multiple sclerosis, in both of which a pathogenic role is postulated for natural killer (NK) cells and natural killer-like T (NK-T) cells. However, there are few studies assessing the role of NK and NK-T cells in psoriasis. We sought to determine whether NK and NK-T cells are present in psoriasis. Skin biopsies were taken from the active edge of a psoriasis plaque and from uninvolved skin at least 5 cm away from involved skin of ten patients with chronic plaque psoriasis. Skin from four normal subjects was used as controls. Using an immunoperoxidase technique, cryostat sections were stained using antibodies to T-cell markers CD2, CD3, CD4 and CD8; cutaneous leucocyte associated antigen; NK cell markers CD16, CD56, CD57, CD94 and CD158a; and the NK-T cell marker CD161. There were significantly more cells expressing T cell markers, NK cell markers CD16, CD57, CD94 and CD158a and NK-T cell marker CD161 in involved skin than in uninvolved or normal skin (P