NEUROPHYSIOLOGY: Dynamic expression of glial cell line-derived neurotrophic factor after cerebral ischemia
NEUROPHYSIOLOGY: Dynamic expression of glial cell line-derived neurotrophic factor after cerebral ischemia
复制标题
神经生理学：脑缺血后胶质细胞源性神经营养因子的动态表达
DOI：
--
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Yi Xue Yuan
中科院分区：
文献类型：
--
作者：
G. Wei;Gen;Xiao;Yi Xue Yuan
本研究旨在通过分子生物学和形态学技术检测胶质细胞源性神经营养因子(GDNF)在大鼠脑缺血损伤中的表达和细胞定位，以了解GDNF在脑缺血损伤中的可能作用。脑缺血再灌流后2 h，梗死灶周围皮质和纹状体区GDNF基因和蛋白的表达开始升高，然后开始下降，72 h后又开始升高，双重染色结果显示GDNF表达的早期高峰为神经元来源，晚期为神经胶质来源。考虑到GDNF的神经营养特性，我们的研究结果提示，内源性GDNF的高表达可能在保护缺血损伤的神经细胞中起重要作用。神经报告11：1177±1183&2000 Lippincott Williams&Wilkins。
The aim of this study was to understand the possible involvement of glial cell line-derived neurotrophic factor (GDNF) in rat brain ischemic injury by examining the expression and the cellular location of GDNF with molecular biological and morphological techniques. Expression of GDNF mRNA and protein was ®rst increased as early as 2 h after ischemia±reperfusion in peri-infarct cerebral cortex and striatum; it then declined, and showed a second increase at 72 h. Double staining con®rmed that the earlier peak of GDNF expression was of neuronal origin and the later peak of glial origin. Considering the neurotrophic characteristics of GDNF, our ®ndings suggest that elevated endogenous GDNF expression may have important roles in protection of ischemic injured neuronal cells. NeuroReport 11:1177±1183 & 2000 Lippincott Williams & Wilkins.