Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardi).
Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardi).
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DOI：
10.1083/jcb.35.3.553
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发表时间：
1967-12
期刊：
影响因子：
--
通讯作者：
Palade GE
中科院分区：
文献类型：
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作者：
Ohad I;Siekevitz P;Palade GE
莱茵衣藻y-1突变体的暗培养细胞含有部分分化的质体，缺少光合层系统。当暴露在光下时，光合作用膜的快速合成伴随着叶绿素、脂类和蛋白质的合成以及淀粉储备的广泛降解。这一过程持续依赖于光照，在没有细胞分裂的情况下，在6-8小时内完成。光合作用活性(放氧、Hill反应、NADP光还原和细胞色素f光氧化)与色素合成和膜形成平行。在绿化过程中，与暗对照相比，与光合作用相关的可溶性酶(RuDP-羧基酶、NADP连接的G-3-P脱氢酶、碱性FDPase(PH 8))的水平只有轻微的变化。此外，细胞色素f浓度在绿化过程中几乎保持不变。叶绿素的合成、光合作用膜的形成和光合作用活性的恢复的动力学表明，这些膜是由它们的成分在一步过程中组装而成的。
Dark-grown cells of the y-1 mutant of Chlamydomonas reinhardi contain a partially differentiated plastid lacking the photosynthetic lamellar system. When exposed to the light, a rapid synthesis of photosynthetic membranes occurs accompanied by synthesis of chlorophyll, lipids, and protein and extensive degradation of the starch reserve. The process is continuously dependent on illumination and is completed within 6–8 hr in the absence of cell division. Photosynthetic activity (O2 evolution, Hill reaction, NADP photo-reduction, and cytochrome f photooxidation) parallels the synthesis of pigment and membrane formation. During the greening process, only slight changes occur in the levels of soluble enzymes associated with the photosynthetic process (RuDP-carboxylase, NADP-linked G-3-P dehydrogenase, alkaline FDPase (pH 8)) as compared with the dark control. Also cytochrome f concentration remains almost constant during the greening process. The kinetics of the synthesis of chlorophyll, formation of photosynthetic membranes, and the restoration of photosynthetic activity suggest that the membranes are assembled from their constituents in a single-step process.