Molecular “light switch” [Ru(phen)2dppzidzo]2+monitoring the aggregation of tau
Molecular “light switch” [Ru(phen)2dppzidzo]2+monitoring the aggregation of tau
复制标题
分子 — 光开关 — [Ru(phen)2dppzidzo]2 监测 tau 聚集
作者：
高星;王丽;黄海量;王林林;姚君亮;石硕;姚天明
监测tau蛋白的聚集是阐明阿尔茨海默病致病机制的关键方案。在本文中，[Ru(phen)2dppzidzo]2+，一种“光开关”钌（II）配合物，被提出作为tau微管结合域的第三个重复单元tau R3肽聚集的新监测探针。与用于染色神经原纤维缠结和监测tau聚集的常用荧光染料硫黄素S相比，该复合物对聚集过程影响较小，固定Stokes位移大，背景发光小，使其成为监测聚集过程和定量检测tau细丝的更好探针。此外，该复合物的长发光寿命也可以扩大其在短寿命荧光背景下检测tau细丝的潜在用途。
Monitoring the aggregation of the tau protein is a key protocol for elucidating the pathogenic mechanism of Alzheimer's disease. In the present article, [Ru(phen)2dppzidzo]2+, a “light switch” ruthenium(II) complex, was presented as a new monitoring probe for the aggregation of a tau R3 peptide, the third repeat unit of the tau microtubule-binding domain. Having little impact on the aggregation process, large fixed Stokes shift and small background luminescence made the complex a better probe for monitoring the aggregation process and quantitatively detecting tau filaments compared to thioflavin S, a commonly used fluorescent dye for staining neurofibrillary tangles and monitoring tau aggregation. Furthermore, a long luminescence lifetime of this complex could also expand its potential usage in the detection of tau filaments in the presence of short-lived fluorescent backgrounds.