A physiologically realistic in vitro model of microvascular networks.
A physiologically realistic in vitro model of microvascular networks.
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DOI：
10.1007/s10544-009-9322-8
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发表时间：
2009-10
影响因子：
2.8
通讯作者：
Kiani, Mohammad F.
中科院分区：
文献类型：
--
作者：
Rosano, Jenna M.;Tousi, Nazanin;Scott, Robert C.;Krynska, Barbara;Rizzo, Victor;Prabhakarpandian, Balabhaskar;Pant, Kapil;Sundaram, Shivshankar;Kiani, Mohammad F.
Existing microfluidic devices, e.g. parallel plate flow chambers, do not accurately depict the geometry of microvascular networks in vivo. We have developed a synthetic microvascular network (SMN) on a polydimethalsiloxane (PDMS) chip that can serve as an in vitro model of the bifurcations, tortuosities, and cross-sectional changes found in microvascular networks in vivo. Microvascular networks from a cremaster muscle were mapped using a modified Geographical Information System, and then used to manufacture the SMNs on a PDMS chip. The networks were cultured with bovine aortic endothelial cells (BAEC), which reached confluency 3-4 days after seeding. Propidium Iodide staining indicated viable and healthy cells showing normal behavior in these networks. Anti-ICAM-1 conjugated 2-μm microspheres adhered to BAEC cells activated with TNF-α in significantly larger numbers compared to control IgG conjugated microspheres. This preferential adherence suggests that cultured cells retain an intact cytokine response in the SMN. This microfluidic system can provide novel insight into characterization of drug delivery particles and dynamic flow conditions in microvascular networks.
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DOI：
10.3109/10739689509148282
发表时间：
1995-01-01
期刊：
Microcirculation (New York)
影响因子：
--
作者：
Frame, Mary D. S.;Sarelius, Ingrid H.
通讯作者：
Sarelius, Ingrid H.
影响因子：
4.8
作者：
Sakhalkar, HS;Hanes, J;Goetz, DJ
通讯作者：
Goetz, DJ
影响因子：
14
作者：
Decuzzi, Paolo;Ferrari, Mauro
通讯作者：
Ferrari, Mauro
影响因子：
2.4
作者：
Prabhakarpandian, B;Goetz, DJ;Kiani, MF
通讯作者：
Kiani, MF
影响因子：
5.5
作者：
Mott, Rosalind E.;Helmke, Brian P.
通讯作者：
Helmke, Brian P.