Procathepsin D secreted by HaCaT keratinocyte cells - A novel regulator of keratinocyte growth.
Procathepsin D secreted by HaCaT keratinocyte cells - A novel regulator of keratinocyte growth.
复制标题
HaCaT 角质形成细胞分泌的组织蛋白酶 D 原 - 角质形成细胞生长的新型调节剂。
DOI：
10.1016/j.ejcb.2007.03.008
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发表时间：
2007
影响因子：
6.6
通讯作者：
Vetvicka,Vaclav
中科院分区：
文献类型：
--
作者：
Vashishta,Aruna;SaraswatOhri,Sujata;Vetvickova,Jana;Fusek,Martin;Ulrichova,Jitka;Vetvicka,Vaclav
Procathepsin D (pCD), the precursor form of lysosomal aspartic protease, is overexpressed and secreted by various carcinomas. The fact that secreted pCD plays an essential role in progression of cancer has been established. In this study, we describe substantial secretion of pCD by the human keratinocyte cell line HaCaT, under serum-free conditions. Moreover, exogenous addition of purified pCD enhanced the proliferation of HaCaT cells. The proliferative effect of pCD was inhibited by a monoclonal antibody against the activation peptide (AP) of pCD. Treatment of HaCaT cells with pCD or AP led to the secretion of a set of cytokines that might promote the growth of cells in a paracrine manner. The role of secreted pCD and its mechanism of action were studied in a scratch wound model and the presence of pCD and AP enhanced regeneration, while this effect was reversed by the addition of anti-AP antibody. Expression and secretion of pCD was upregulated in HaCaT cells exposed to various stress conditions. Taken together, our results strongly suggest that the secretion of pCD is not only linked to cancer cells but also plays a role in normal physiological conditions like wound healing and tissue remodeling.
