Full reconstruction of large lobula plate tangential cells in Drosophila from a 3D EM dataset
Full reconstruction of large lobula plate tangential cells in Drosophila from a 3D EM dataset
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DOI：
10.1371/journal.pone.0207828
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发表时间：
2018-11-28
期刊：
影响因子：
3.7
通讯作者：
Borst, Alexander
中科院分区：
文献类型：
--
作者：
Boergens, Kevin M.;Kapfer, Christoph;Borst, Alexander
随着神经遗传学方法的出现，行为的神经基础目前正在被越来越详细地分析。对于黑腹果蝇的视觉驱动行为尤其如此，在这种行为中，存在细胞特有的驱动系，根据与适当的效应基因的结合，允许对个别细胞类型进行定向记录、沉默和光遗传刺激。再加上苍蝇视叶大部分的详细连接数据，这使得我们最近在理解局部运动检测的神经回路方面取得了很大进展。然而，这种局部信息是如何被视流敏感的大视野神经元结合起来的，目前仍不完全清楚。在这里，我们的目标是通过对苍蝇小叶板切向细胞的致密重建来填补这一空白。这些神经元从数百个局部运动感知T4/T5神经元收集信息，并将它们连接到下行神经元或中央大脑区域。我们证实了先前从光学显微镜发表的HS和VS细胞的所有基本特征。此外，我们还发现了背侧和腹侧的离心性水平、DCH和VCH细胞，以及三个VS样细胞，包括它们明显的树突和轴突投射区域。
With the advent of neurogenetic methods, the neural basis of behavior is presently being analyzed in more and more detail. This is particularly true for visually driven behavior of Drosophila melanogaster where cell-specific driver lines exist that, depending on the combination with appropriate effector genes, allow for targeted recording, silencing and optogenetic stimulation of individual cell-types. Together with detailed connectomic data of large parts of the fly optic lobe, this has recently led to much progress in our understanding of the neural circuits underlying local motion detection. However, how such local information is combined by optic flow sensitive large-field neurons is still incompletely understood. Here, we aim to fill this gap by a dense reconstruction of lobula plate tangential cells of the fly lobula plate. These neurons collect input from many hundreds of local motion-sensing T4/T5 neurons and connect them to descending neurons or central brain areas. We confirm all basic features of HS and VS cells as published previously from light microscopy. In addition, we identified the dorsal and the ventral centrifugal horizontal, dCH and vCH cell, as well as three VSlike cells, including their distinct dendritic and axonal projection area.