Cytokine secretion in vivo and ex vivo following chemotherapy of Mycobacterium tuberculosis infection
Cytokine secretion in vivo and ex vivo following chemotherapy of Mycobacterium tuberculosis infection
复制标题
DOI：
10.1016/s0035-9203(96)90141-8
复制
发表时间：
1996-03-01
影响因子：
2.2
通讯作者：
Griffin, GE
中科院分区：
文献类型：
--
作者：
Friedland, JS;Hartley, JC;Griffin, GE
人类对结核病的免疫反应部分是由促炎性细胞因子肿瘤坏死因子(肿瘤坏死因子)、白介素6和白介素8介导的。我们研究了赞比亚患者全血白细胞体外最大内毒素刺激前后血浆中这些细胞因子的浓度。对32例非致死性肺结核患者进行了9个月的随访，其中25例人类免疫缺陷病毒(HIV)血清阳性。患者在入院时(访问A)、抗分枝杆菌治疗2个月后(访问B)和化疗结束时(访问C)进行评估。在A和B两次访问之间，患者体重恢复(P=0.0001.0 3)，贫血减轻(P=0.0 5)。在B次访问中，艾滋病毒血清阳性患者的血红蛋白浓度仍然较低(P=0.002)，而最初在所有患者中升高的血沉在血清阴性患者1小时内升高(100±6 mm比43+/-11 mm；P=0001)。C组血浆IL-8浓度和体外IL-8分泌量均显著高于C组(P
The human immune response to tuberculosis is partly mediated by the proinflammatory cytokines tumour necrosis factor (TNF), interleukin (IL)-6, and IL-8. We investigated plasma concentrations of these cytokines before and after maximal lipopolysaccharide stimulation ex vivo of whole blood leucocytes from Zambian patients. 32 patients with non-fatal tuberculosis, 25 of whom were seropositive for human immunodeficiency virus (HIV), were followed for 9 months. Patients were assessed at presentation to hospital (visit A), after 2 months' antimycobacterial therapy (visit B), and when chemotherapy was completed (visit C). Between visits A and B, patients regained weight (P=0 03) and became less anaemic (P=0.0001). At visit B, haemoglobin concentration remained lower in HIV seropositive patients (P=0 001) and the erythrocyte sedimentation rate (ESR), initially elevated in all patients, was higher in HIV seropositive patients (100+6 mm vs. 43+/-11 mm in 1 h in seronegative patients; P=0.002). Plasma IL-8 concentrations were increased at visit C as was IL-8 secretion ex vivo (P