FUNCTIONAL-ALPHA AND BETA-T-CELL CHAIN RECEPTOR MESSAGES CAN BE DETECTED IN OLD BUT NOT IN YOUNG ATHYMIC MICE
FUNCTIONAL-ALPHA AND BETA-T-CELL CHAIN RECEPTOR MESSAGES CAN BE DETECTED IN OLD BUT NOT IN YOUNG ATHYMIC MICE
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DOI：
10.1002/eji.1830170407
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发表时间：
1987-04-01
影响因子：
5.4
通讯作者：
NOMOTO, K
中科院分区：
文献类型：
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作者：
KISHIHARA, K;YOSHIKAI, Y;NOMOTO, K
T细胞抗原受体(TCR)α的表达和序列分析和β-链基因在先天性无性体(裸鼠)小鼠的脾中进行了研究。少量Thy-1+细胞(.apprx.在幼年(8周龄)裸鼠的尼龙毛浓缩脾细胞中检测到L3T4和Lyt-2表面抗原，未检测到L3T4和Lyt-2表面抗原。这些裸鼠只表达低水平的1.0kbβ链mRNA和很少或没有α基因转录本。老年(20周龄)裸鼠Thy-1+细胞略有增加(24%)，可检测到少量L3T4+细胞(10%)和/或Lyt-2+细胞(5%)。“全长”1.7kb的α链和1.3kb的β链信息可以在来自老年裸鼠的富含尼龙羊毛的脾细胞中发现。对该基因的序列分析表明，该基因没有功能性α。在幼年无瘤小鼠的脾细胞中可以检测到β-链基因，而一些nu/nu基因型的老年小鼠则由完全重排的V-(D)-J-C-基因片段组成，这些基因片段编码潜在的功能蛋白。有趣的是，三个独立的编码α的cDNA克隆中的两个。Chain使用了相同的V.Alpha。和J.Alpha基因片段。这些结果表明，胸腺外TCR.α。而β链基因重排确实在裸鼠体内发生，尽管在一定程度上是缓慢的，可能是这些老年无胸腺小鼠有限的抗原和/或与H-2相关的T细胞功能的原因。数据进一步表明，无菌小鼠的TCR谱系也可能是有限的。
The expression and sequences of T cell antigen receptor (TcR) .alpha. and .beta.-chain genes were investigated in the spleens from congenitally athymic (nude) mice. A small number of Thy-1+ cells (.apprx. 7.0%) was found in nylon wool-enriched spleen cells from young (8 weeks old) nude mice but no L3T4 or Lyt-2 surface antigens were detected. These nude mice expressed only a low level of 1.0-kb .beta.-chain mRNA and little or no .alpha.-gene transcripts. On the other hand the number of Thy-1+ spleen cells increased slightly (to 24%) in old (20 weeks old) nude mice and a small number of L3T4+ (10%) and/or Lyt-2+ (5%) cells were detectable. "Full length" 1.7 kb .alpha.-chain and 1.3-kb .beta.-chain messages could be found in nylon wool-enriched spleen cells from old nude mice. Sequence analysis of the cDNA revealed that no functional .alpha. and .beta.-chain genes can be detected in the spleen cells of young athymic mice while some of the old mice with nu/nu genotype are composed of completely rearranged V-(D)-J-C-gene segments which encode potentially functional proteins. Interesingly, two of three independent cDNA clones encoding the .alpha. chain used the same V.alpha. and J.alpha.-gene segments. These results suggest that extrathymic TcR .alpha. and .beta.-chain gene rearrangements do occur, though slowly, to some extent, in nude mice and may be responsible for the limited antigen and/or H-2-related T cell functions in these old athymic mice. The data further suggests that the TcR repertoire in athymic mice may also be limited.