PRODUCTION OF ARTIFICIAL-ORIENTED MATS AND STRANDS FROM PLASMA FIBRONECTIN - A MORPHOLOGICAL-STUDY
PRODUCTION OF ARTIFICIAL-ORIENTED MATS AND STRANDS FROM PLASMA FIBRONECTIN - A MORPHOLOGICAL-STUDY
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DOI：
10.1016/0142-9612(93)90038-4
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发表时间：
1993-08-01
期刊：
影响因子：
14
通讯作者：
BROWN, RA
中科院分区：
文献类型：
--
作者：
EJIM, OS;BLUNN, GW;BROWN, RA
纤连蛋白是一种参与伤口愈合的细胞外基质糖蛋白。已经开发出从血浆纤连蛋白制备定向纤连蛋白垫的技术，用于伤口愈合和组织修复。线和垫的形成是通过溶液中纤连蛋白在定向剪切力下的自缔合和聚集而形成的。肝素与纤连蛋白的结合对垫进行了改性。扫描电子显微镜显示，这些垫由定向良好的纤维状纤连蛋白组成，具有内部互连的孔网络。制备单链纤连蛋白用作细胞与聚集定向纤连蛋白垫相互作用的组织培养模型。大鼠高肌腱和人皮肤成纤维细胞用于评估细胞与单链纤连蛋白和垫的相互作用。成纤维细胞迅速附着在单链上并定向。根据下面的纤连蛋白的方向，在单链上生长的成纤维细胞的致密培养物变得定向。成纤维细胞很容易在纤连蛋白垫的表面上生长，并在多孔网络内观察到。
Fibronectin is an extracellular matrix glycoprotein involved in wound healing. Techniques have been developed for the preparation of orientated fibronectin mats from plasma fibronectin, for use in wound healing and tissue repair. Formation of strands and mats is by self-association and aggregation of fibronectin from solution under a directional shear force. Incorporation of heparin with fibronectin modified the mats. Scanning electron microscopy showed that the mats were composed of well-orientated fibrous fibronectin, with a network of internal interconnecting pores. Single strands of fibronectin were prepared for use as a tissue culture model of cell interaction with aggregated orientated fibronectin mats. Rat tall tendon and human skin fibroblasts were used for assessment of cell interaction with both single strands of fibronectin and mats. Fibroblasts rapidly attached to single strands and became orientated. Dense cultures of fibroblasts growing over single strands became orientated, according to the orientation of the underlying fibronectin. Fibroblasts readily grew on the surface of fibronectin mats and were observed within the porous network.