Autophagy-related lipase FgATG15 of Fusarium graminearum is important for lipid turnover and plant infection
Autophagy-related lipase FgATG15 of Fusarium graminearum is important for lipid turnover and plant infection
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DOI：
10.1016/j.fgb.2010.11.004
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发表时间：
2011-03-01
影响因子：
3
通讯作者：
Schaefer, Wilhelm
中科院分区：
文献类型：
--
作者：
Long Nam Nguyen;Bormann, Joerg;Schaefer, Wilhelm
自噬是真核细胞中的一种非选择性降解途径，从酵母到人类都是保守的。自噬参与了几种病原真菌的毒力，如稻瘟病菌或轮纹炭疽菌。在目前的研究中，我们鉴定并干扰了禾谷镰刀菌中一种类似自噬的脂肪酶FgATG15。结果表明，FgATG15在巴斯德毕赤酵母中异源表达时具有脂肪酶活性。我们使用基因缺失的方法来表征该酶的功能。我们证明FgATG15参与真菌生长和气生菌丝的产生。FgATG15还参与分生孢子的产生和萌发，破坏FgATG15导致分生孢子形状异常。在饥饿条件下，FgATG15的破坏物质减少了贮藏脂肪的降解，暗示FgATG15的S参与了脂肪的周转。此外，这些干扰物对小麦穗部的侵染严重减弱，表明FgATG15参与了小麦的致病过程。此外，我们还发现FgATG15干扰物的脱氧雪腐镰刀菌烯醇水平显著低于野生型菌株。综上所述，我们表明FgATG15参与了许多发育过程，可以作为抗真菌的靶点。(C)2010 Elsevier Inc.保留所有权利。
Autophagy is a non-selective degradation pathway in eukaryotic cells that is conserved from yeasts to humans. Autophagy is involved in the virulence of several pathogenic fungi such as Magnaporthe grisea or Colletotrichum orbiculare. In the current study, we identified and disrupted an autophagy-like lipase FgATG15 in Fusarium graminearum. We showed that FgATG15 exhibits lipase activity when heterologously expressed in P. pastoris. We used a gene deletion approach to characterize the function of the enzyme. We demonstrate that FgATG15 is involved in fungal growth and aerial hyphae production. FgATG15 is also involved in conidia production and germination, and disruption of FgATG15 led to aberrant conidia shapes. FgATG15 disruptants were reduced in storage lipid degradation under starvation conditions, implicating FgATG15's involvement in lipid turnover. Moreover, wheat head infection by the disruptants was severely attenuated, indicating the involvement of FgATG15 in pathogenesis. Additionally, we found that the deoxynivalenol levels of FgATG15 disruptants were significantly decreased compared with the wild type strain. Taken together, we show that FgATG15 is involved in numerous developmental processes and could be exploited as an antifungal target. (C) 2010 Elsevier Inc. All rights reserved.