Molecular epidemiology of bovine anaplasmosis in Khyber Pakhtunkhwa, Pakistan
Molecular epidemiology of bovine anaplasmosis in Khyber Pakhtunkhwa, Pakistan
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DOI：
10.1007/s11250-018-1599-2
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发表时间：
2018-10-01
影响因子：
1.7
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
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作者：
Farooqi, Shahid Hussain;Ijaz, Muhammad;Zhang, Hui
牛无形体病是巴基斯坦的地方病，它降低了牲畜的生产力，导致高死亡率，特别是在幼龄动物中。这项研究的目的是确定潜在的风险因素负责的发生和传播的无形体病的牛和水牛在巴基斯坦的第一次。共收集了900（牛=479，布法罗=421）血液样本，无论年龄和性别从三个不同的开伯尔-普赫图赫瓦（KP）省的巴基斯坦。应用聚合酶链反应（PCR）技术对无形体病进行分子检测。在试点问卷上收集的数据包括11个预测变量，使用R-统计软件进行分析，并使用单变量分析评估因变量和自变量之间的关联。采用了自动和人工方法，产生了可比较的模型。在所有方法中确定的关键风险因素包括动物种属、动物品种、动物性别、蜱虫感染状态、既往蜱虫史、蜱虫控制状态和所用杀螨剂（比值比>1）。通过PCR扩增无形体属16 SrRNA基因的611 bp特异性片段。是从165个样本中产生的。通过测序和BLAST查询确认样品的无形体病。目前研究的结果得出结论，通过加强保护措施，控制已确定的风险因素，可以减少无形体病在巴基斯坦的传播。
Bovine anaplasmosis is endemic in Pakistan where it reduces livestock productivity and leads to high mortality, especially in young animals. This study was aimed to identify the potential risk factors responsible for the occurrence and spread of anaplasmosis in cattle and buffaloes for the first time in Pakistan. A total of 900 (cattle=479, buffalo=421) blood samples were collected irrespective of age and sex from three distinct zones of Khyber Pakhtunkhhwa (KP) province of Pakistan. Polymerase chain reaction (PCR) technique was used for the molecular detection of anaplasmosis. Data collected on a piloted questionnaire including 11 predicting variables which were analyzed using R-statistical software, and association between the dependent and independent variables was assessed using univariable analysis. Automated and manual approaches were exercised, producing comparable models. Key risk factors identified in all the approaches included species of the animal, breed of animal, sex of animal, tick infestation status, previous tick history, tick control status, and acaricides used (odds ratio>1). The 611bp DNA fragment specific for 16S rRNA gene of Anaplasma spp. was produced from 165 samples. The samples were confirmed for anaplasmosis through sequencing and BLAST queries. The findings of the current study conclude that by enhancing the protective measures to control the identified risk factors can reduce the spread of anaplasmosis in Pakistan.