Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)
Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)
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DOI：
10.1099/vir.0.030338-0
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发表时间：
2011-06-01
影响因子：
3.8
通讯作者：
Varsani, Arvind
中科院分区：
文献类型：
--
作者：
Rosario, Karyna;Marinov, Milen;Varsani, Arvind
蜻蜓环状病毒（DfCyV），一种新的ssDNA病毒，使用病毒宏基因组学在来自汤加王国的蜻蜓（天蛾科）中发现。DfCyV的宏基因组序列与圆环病毒科中最近提出的圆环病毒属的病毒相似。特异性PCR导致从三种昆虫（Pantala flavescens、Tholymis tillarga和Diplacodes bipunctata）中回收21个DfCyV基因组。DfCyV基因组全长1741 nt，核苷酸同源性>95%，可分为11个亚型。DfCyV基因组与人类粪便样品中鉴定的环状病毒具有48-63%的全基因组核苷酸同一性。重组分析揭示了三个重组DfCyV基因组，表明重组在环状病毒进化中起着重要作用。据我们所知，这是第一次在昆虫中发现环状ssDNA病毒，这些数据可能有助于阐明最近在动物和环境样本中发现的新型圆环病毒科之间的进化联系。
Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (family Libellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1 741 nt DfCyV genomes share >95% nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48-63% genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.