A novel method for precisely timed stimulation of mouse whiskers in a freely moving preparation: Application for delivery of the conditioned stimulus in trace eyeblink conditioning
A novel method for precisely timed stimulation of mouse whiskers in a freely moving preparation: Application for delivery of the conditioned stimulus in trace eyeblink conditioning
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DOI：
10.1016/j.jneumeth.2008.11.002
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发表时间：
2009-03-15
影响因子：
3
通讯作者：
Disterhoft, John
中科院分区：
文献类型：
--
作者：
Galvez, Roberto;Weiss, Craig;Disterhoft, John
体感胡须通路是一个有用的系统，可以增加我们对经验诱发的可塑性的理解。然而，在清醒的自由移动小鼠中精确定时激活胡须是非常困难的。该手稿描述了一种构建胡须刺激器的方法，该刺激器可以连接到自由移动的鼠标上。刺激器用于在时间敏感的前脑依赖性任务中激活胡须，即微量眨眼调节。在无刺激的跟踪间隔后，反复将胡须刺激与温和的眼眶周围电击配对后，跟踪条件小鼠能够了解这种关联。这项研究证明了在时间敏感的行为任务中使用胡须刺激器的潜力，例如微量眨眼条件反射，从而增强我们检查清醒行为啮齿动物体感胡须通路中经验诱导的神经元可塑性的能力。由 Elsevier B.V. 出版
The somatosensory whisker pathway has been a useful system for increasing our understanding of experience-induced plasticity. However, precisely timed whisker activation in the awake freely moving mouse has been very difficult. This manuscript describes a method for construction of a whisker stimulator that can be attached to a freely moving mouse. The stimulator was used to activate the whiskers in a time-sensitive forebrain-dependent task, trace eyeblink conditioning. After repeatedly pairing whisker stimulation with delivery of a mild periorbital shock following a stimulus-free trace interval, trace-conditioned mice were able to learn the association. This study demonstrates the potential for using the whisker stimulator in time-sensitive behavioral tasks, such as trace eyeblink conditioning, thus enhancing our ability to examine experience-induced neuronal plasticity in the somatosensory whisker pathway in awake behaving rodents. Published by Elsevier B.V.