Selective separation and purification of two lipases from chromobacterium viscosum using AOT reversed micelles
Selective separation and purification of two lipases from chromobacterium viscosum using AOT reversed micelles
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DOI：
10.1002/bit.260381107
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发表时间：
1991-12
影响因子：
3.8
通讯作者：
M. Aires-Barros;J. Cabral
中科院分区：
文献类型：
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作者：
M. Aires-Barros;J. Cabral
使用反胶束系统通过液-液萃取对来自粘性色杆菌的两种脂肪酶进行选择性分离和纯化。使用异辛烷中的 250 mM AOT 胶束溶液确定最佳提取参数。使用含有 50 mM KCl 的 50 mM 磷酸钾缓冲溶液在 pH 6.0 下实现两种脂肪酶的完全分离。通过向负载脂肪酶的胶束溶液中添加 2.5%（体积）的乙醇，可以在 pH 9.0 的新水相（50 mM K2HPO4 和 50 mM KCl）中回收 85% 的提取脂肪酶。脂肪酶 A 纯化 2.6 倍，回收率为 86%，脂肪酶 B 纯化 1.5 倍，回收率为 76%。
Selective separation and purification of two lipases form Chromobacterium viscosum were carried out by liquid‐liquid extraction using a reversed micellar system. Optimum parameters for extraction were determined using a 250 mM AOT micellar solution in isooctane. Complete separation of the two lipases was achieved at pH 6.0 with a 50mM potassium phosphate buffer solution containing 50 mM KCI. By adding 2.5% by volume of ethanol to the lipase‐loaded micellar solution, 85% of the extracted lipase could be recovered in a new aqueous phase, 50 mM K2HPO4 with 50 mM KCl, at pH 9.0. Lipase A was purified 2.6‐fold with a recovery of 86%, and lipase B by 1.5‐fold with a recovery of 76%.