Quantitative assessment of early liver fibrosis in rats using N-13-NH3 center dot H2O PET/CT
Quantitative assessment of early liver fibrosis in rats using N-13-NH3 center dot H2O PET/CT
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13N-NH3·H2O PET/CT定量评估大鼠早期肝纤维化
DOI：
10.1097/mnm.0000000000000415
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发表时间：
2016
影响因子：
1.5
通讯作者：
Xin Jun
中科院分区：
文献类型：
--
作者：
Han Ting-Ting;Du Ming;Zhang Xin;Cao Li;Li Hong;Zhao Zhou-She;Guo Qi-Yong;Xin Jun
目的探讨13 N NH3·H2 O PET/CT及乙酰唑胺（Acetazolamide，ACZ）对早期肝纤维化的定量诊断及肝纤维化分期的价值。以13 N-NH3·H2O为显像剂，ACZ为水通道蛋白1抑制剂，对大鼠进行45 min的PET/CT动态扫描，根据数据重建和图像分析，计算平均标准摄取值在20、90 s、5、10、15、20和25 min的时间点，比较对照组和肝纤维化模型组不同时间点的肝脏SUV均值。比较两组大鼠的血液灌注、组织间弥散和13 N-NH3·H2O代谢情况。单因素方差分析被用来分析各组之间的SUV在任何时间点的差异和配对样本t-检验被用来比较SUV之前和之后，除了inhibitors.ResultsWe包括4只大鼠在对照组（S 0）和20只大鼠在肝纤维化组（其中包括早期S1：11只大鼠，进行性阶段S2+ S3+ S4：9只大鼠）。S 0中的3只大鼠、S1中的7只大鼠和S2+ S3+ S4中的6只大鼠配对。90 s时的SUV均值差异有统计学意义（P< 0.05），而其他组在所有时间点均存在统计学差异（P> 0.05）。加入抑制剂ACZ后，10、15、20、25 min时S 0、S2+ S3+ S4的SUV均值差异有统计学意义（P< 0.05）。结论13 N-NH3·H2O PET/CT可辅助肝纤维化的诊断。13 N-NH3·H2O PET/CT联合ACZ检查有助于鉴别肝纤维化的早期和进展期。
ObjectiveTo investigate the value of 13 N-NH 3· H 2 O PET/computed tomography (CT) and the inhibitor, acetazolamide (ACZ), in the quantitative diagnosis of early liver fibrosis and the assessment of liver fibrosis stage.Materials and methodsTwenty-four male Wistar rats (293.54±37.99 g) were used in this study and grouped by pathology after biopsy. Using 13 N-NH 3· H 2 O as an imaging agent and ACZ as an aquaporin 1 inhibitor, the rats were subjected to a dynamic PET/CT scan for 45 min. According to data reconstruction and imaging analysis, we calculated the mean standard uptake value (SUV mean) of the liver at the time points of 20, 90 s, 5, 10, 15, 20, and 25 min. From the results of 13 N-NH 3· H 2 O, the liver SUV mean in the control group and the liver fibrosis model group at different time points were compared. In addition, blood perfusion, interstitial diffusion, and 13 N-NH 3· H 2 O metabolism of the two groups were compared. Single-factor analysis of variance was used to analyze the differences between the SUVs in the groups at any time point and the paired-sample t-test was used to compare the SUVs before and after addition of the inhibitor.ResultsWe included four rats in the control group (S0) and 20 rats in the liver fibrosis group (which included early-stage S1: 11 rats, progressive-stage S2+ S3+ S4: nine rats). Three rats in S0, seven rats in S1, and six rats in S2+ S3+ S4 were paired. The SUV mean at 90 s was statistically different (P< 0.05); however, the other groups showed statistical difference at all time points (P> 0.05). After the addition of the inhibitor, ACZ, the SUV mean of S0 and S2+ S3+ S4 at 10, 15, 20, and 25 min was statistically different (P< 0.05).Conclusion13 N-NH 3· H 2 O PET/CT may aid the diagnosis of liver fibrosis. 13 N-NH 3· H 2 O PET/CT combined with ACZ may help distinguish between the early stage and the progressive stage of liver fibrosis.