Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans.
Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans.
复制标题
人类抗阻运动后肌肉蛋白质周转率和氨基酸转运率增加。
DOI：
10.1152/ajpendo.1995.268.3.e514
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Wolfe,RR
中科院分区：
文献类型：
--
作者：
Biolo,G;Maggi,SP;Williams,BD;Tipton,KD;Wolfe,RR
测定未经训练的吸收后正常志愿者小腿肌肉蛋白质合成、降解和氨基酸转运的速率。方法包括使用稳定的氨基酸同位素示踪剂，股血管的动静脉插管，以及股外侧肌的活组织检查。在运动后恢复期间，肌肉内苯丙氨酸用于蛋白质合成的利用率比基础值增加了108+/-18%，而蛋白质分解的释放率增加了51+/-17%。肌肉蛋白质平衡在运动后有所改善(P<；0.05)，但并未转为正值(从-15+/-12降至-6+/-3nmol苯丙氨酸。分钟-1.100毫升腿部体积-1)。运动后，亮氨酸、赖氨酸和丙氨酸的内向转运速率在基础状态之上分别从132+/-16增加到208+/-29，从122+/-8增加到260+/-8，从384+/-71增加到602+/-89nmol.min-1.100 mlLeg-1。苯丙氨酸的转运没有明显变化。这些结果表明，在耐力运动后的恢复过程中，由于肌肉蛋白质合成和降解的加速，肌肉蛋白质的周转增加。运动后氨基酸转运的加速可能有助于相对较大的蛋白质合成刺激。
The rates of protein synthesis and degradation and of amino acid transport were determined in the leg muscle of untrained postabsorptive normal volunteers at rest and approximately 3 h after a resistance exercise routine. The methodology involved use of stable isotopic tracers of amino acids, arteriovenous catheterization of the femoral vessels, and biopsy of the vastus lateralis muscle. During postexercise recovery, the rate of intramuscular phenylalanine utilization for protein synthesis increased above the basal value by 108 +/- 18%, whereas the rate of release from proteolysis increased by 51 +/- 17%. Muscle protein balance improved (P < 0.05) after exercise but did not become positive (from -15 +/- 12 to -6 +/- 3 nmol phenylalanine.min-1.100 ml leg volume-1). After exercise, rates of inward transport of leucine, lysine, and alanine increased (P < 0.05) above the basal state from 132 +/- 16 to 208 +/- 29, from 122 +/- 8 to 260 +/- 8, and from 384 +/- 71 to 602 +/- 89 nmol.min-1.100 ml leg-1, respectively. Transport of phenylalanine did not change significantly. These results indicate that, during recovery after resistance exercise, muscle protein turnover is increased because of an acceleration of synthesis and degradation. A postexercise acceleration of amino acid transport may contribute to the relatively greater stimulation of protein synthesis.