The identification of nuclear factor Akirin with immune defense role in silkworm, Bombyx mori
The identification of nuclear factor Akirin with immune defense role in silkworm, Bombyx mori
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家蚕核因子 Akirin 的免疫防御作用的鉴定
DOI：
10.1016/j.ijbiomac.2021.07.193
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发表时间：
2021-08-06
影响因子：
8.2
通讯作者：
Cui，Hongjuan
中科院分区：
文献类型：
--
作者：
Hu，Xin;Zhang，Kui;Cui，Hongjuan
Akirins是一种高度保守的核因子，调节多种生理过程，如先天免疫。Akirins在脊椎动物和许多无脊椎动物中的生物学功能已被广泛研究，但到目前为止还没有关于鳞翅目昆虫的报道。在本研究中，我们从家蚕中鉴定并鉴定了一种新的Akirin（命名为BmAkirin），并探讨了其在天然免疫中的潜在作用。结果表明，BmAkirin在各组织中的表达水平不一致，但在睾丸中的转录水平最高，其次是卵巢和血细胞。它在胚胎发育的早期阶段也有显著的表达水平。在病毒、真菌、革兰氏阴性菌和革兰氏阳性菌的攻击下，脂肪体和血细胞中BmAkirin的表达均不同程度地增加。免疫荧光分析显示BmAkirin蛋白主要定位于细胞核。BmAkirin基因的敲低可显著降低AMP的表达，降低幼虫在免疫刺激下的存活能力。此外，BmAkirin耗竭后，幼虫的细菌清除能力也下降。以上结果表明，BmAkirin在家蚕（B. mori）通过在体内正向调节AMP表达。
Akirins, highly conserved nuclear factors, regulate diverse physiological processes such as innate immunity. The biological functions of Akirins have extensively been studied in vertebrates and many invertebrates; however, there is no report so far on lepidopteran insects. In the present study, we identified and characterized a novel Akirin from the silkworm,Bombyx mori(designated asBmAkirin), and explored its potential roles in innate immunity. The expression analysis revealed the unequal mRNA levels ofBmAkirinin all the tested tissues; however, the gene's transcription level was highest in testis, followed by ovaries and hemocytes. It also had significant expression levels at the early stages of embryonic development. Expression ofBmAkirinin fat bodies and hemocytes exhibited an increase in various degrees when challenged with virus, fungus, Gram-negative bacteria, and Gram-positive bacteria. Immunofluorescence analysis showed BmAkirin protein was prominently localized in the nucleus. Knockdown ofBmAkirinstrongly reduced the expression of AMPs and decreased the survival ability of larva upon immune stimulation. Moreover, the bacterial clearance ability of larvae was also decreased following the depletion ofBmAkirin. Collectively, our results demonstrate thatBmAkirinplays an indispensable role in the innate immunity ofBombyx mori(B. mori)by positively modulating AMPs expression in vivo.