Air-dried cells from the anhydrobiotic insect, Polypedilum vanderplanki, can survive long term preservation at room temperature and retain proliferation potential after rehydration
Air-dried cells from the anhydrobiotic insect, Polypedilum vanderplanki, can survive long term preservation at room temperature and retain proliferation potential after rehydration
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DOI：
10.1016/j.cryobiol.2016.05.006
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发表时间：
2016-08-01
期刊：
影响因子：
2.7
通讯作者：
Okuda, Takashi
中科院分区：
文献类型：
--
作者：
Watanabe, Kazuyo;Imanishi, Shigeo;Okuda, Takashi
Pv11是一种来源于无水生物昆虫的细胞系，在室温下以干燥形式(仅6%残余水分)保存长达251天，复水后重新开始增殖。之前的一项研究已经报道了Pv11细胞在干燥后存活，但没有随后的增殖。在此，对该方案进行了改进，以提高Pv11细胞在干保存后的存活率和增殖能力。该方法主要包括预孵化、脱水和复水过程，并对每一步进行了研究。到目前为止，在600 mM海藻糖溶液中预培养48h再脱水是成功实现Pv11细胞干保存的最有利的预适应，使复水和随后的细胞增殖后的存活率达到约16%。尽管本文建立的Pv11细胞的简单风干方法不适用于其他昆虫细胞系的干燥保存，但Pv11是第一个可干燥保存的动物细胞系，不仅将为基础科学做出贡献，而且将为应用科学做出贡献。(C)2016年提交人。由爱思唯尔公司出版。
Pv11, a cell line derived from the anhydrobiotic insect, Polypedilum vanderplanki, was preserved in a dry form (only 6% residual moisture) at room temperature for up to 251 days and restarted proliferating after rehydration. A previous study already reported survival of Pv11 cells after desiccation, but without subsequent proliferation. Here, the protocol was improved to increase survival and achieve proliferation of Pv11 cells after dry storage. The method basically included preincubation, desiccation and rehydration processes and each step was investigated. So far, preincubation in a 600 mM trehalose solution for 48 h before dehydration was the most favourable preconditioning to achieve successful dry preservation of Pv11 cells, allowing about 16% of survival after rehydration and subsequent cell proliferation. Although the simple air-dry method established for Pv11 cells here was not applicable for successful dry preservation of other insect cell lines, Pv11 is the first dry-preservable animal cell line and will surely contribute not only to basic but also applied sciences. (C) 2016 The Authors. Published by Elsevier Inc.